Description

5'-O-(Dimethoxytrityl)-N3/O4-(Toluoyl)-2-Thiothymidine is a sophisticated, protected nucleoside derivative essential for the synthesis of modified oligonucleotides. This compound is a critical building block in advanced pharmaceutical research, particularly for the development of therapeutic nucleic acids and diagnostic probes. It is primarily utilized by research institutions, biotechnology firms, and pharmaceutical companies engaged in antisense, siRNA, and aptamer development.

Application

• Oligonucleotide Synthesis: Serves as a key phosphoramidite precursor for the solid-phase synthesis of oligonucleotides containing 2-thiothymidine modifications.
• Antisense & siRNA Therapeutics: Used in the research and development of gene-silencing drugs where enhanced nuclease resistance and binding affinity are required.
• Diagnostic Probe Development: Enables the creation of high-specificity probes for molecular diagnostics and bioanalytical assays.
• Structural Biology Studies: Employed to investigate nucleic acid structure, function, and protein-nucleic acid interactions.
• Aptamer Engineering: A vital component in the synthesis of modified aptamers with improved stability and target affinity for therapeutic and sensing applications.
• Academic & Industrial R&D: Fundamental reagent for chemical biology and medicinal chemistry research programs focused on nucleic acid technology.

Basic Information

Product Name	5'-O-(Dimethoxytrityl)-N3/O4-(Toluoyl)-2-Thiothymidine
CAS No.	156783-21-0
Molecular Formula	C40H38N2O7S
Molecular Weight	690.81 g/mol
Synonyms	5'-O-DMT-N3/O4-Toluoyl-2-thiothymidine; 2-Thiothymidine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-N3,O4-bis(4-methylbenzoyl)-; DMT-T(sT)-OH; 2'-Deoxy-2-thiothymidine, 5'-O-(4,4'-dimethoxytrityl)-N3,O4-di-p-toluoyl-; Protected 2-Thiothymidine; 5'-O-(4,4'-Dimethoxytrityl)-2-thiothymidine N3,O4-bis(p-toluoyl) ester
EINECS	Contact for details

Quality Control

Our production of 5'-O-(Dimethoxytrityl)-N3/O4-(Toluoyl)-2-Thiothymidine adheres to stringent in-process controls to ensure batch-to-batch consistency and high purity, suitable for sensitive pharmaceutical research. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We are committed to meeting the exacting standards required for GMP-grade starting materials in oligonucleotide synthesis.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, consider storing under an inert atmosphere such as argon or nitrogen.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.