Description

5'-O-(Dimethoxytrityl)-N3/O4-(Toluoyl)-2-Thiothymidine is a sophisticated nucleoside derivative, a key protected intermediate in the synthesis of modified oligonucleotides. This compound is critical for researchers and manufacturers developing therapeutic nucleic acids, such as antisense oligonucleotides and siRNA, where precise chemical modification is required to enhance stability and efficacy. It is primarily utilized by pharmaceutical R&D departments, biotechnology companies, and specialized CROs/CMOs engaged in advanced oligonucleotide synthesis.

Application

• Oligonucleotide Synthesis: Serves as a vital phosphoramidite precursor for the solid-phase synthesis of oligonucleotides containing 2-thiothymidine modifications.
• Antisense Drug Development: Used in the research and production of antisense oligonucleotides (ASOs) designed for targeted gene silencing therapies.
• siRNA and RNAi Therapeutics: A key building block for creating chemically stabilized small interfering RNA (siRNA) constructs with improved nuclease resistance and pharmacokinetic profiles.
• Diagnostic Probe Development: Employed in the manufacture of labeled nucleic acid probes for diagnostic assays and genomic research.
• Medicinal Chemistry Research: Acts as a specialized monomer for exploring structure-activity relationships (SAR) in novel nucleoside-based drug candidates.
• Academic & Institutional Research: Supports fundamental studies in nucleic acid chemistry, biochemistry, and molecular biology within university and government laboratories.

Basic Information

Product Name	5'-O-(Dimethoxytrityl)-N3/O4-(Toluoyl)-2-Thiothymidine
CAS No.	156783-13-0
Molecular Formula	C41H38N2O7S
Molecular Weight	702.82 g/mol
Synonyms	2'-Deoxy-2-thiothymidine, 5'-O-DMT, N3,O4-bis(toluoyl) derivative; 5'-O-(4,4'-Dimethoxytrityl)-2-thiothymidine N3,O4-bis(4-methylbenzoyl); DMT-2-Thiothymidine(toluoyl); 2-Thiothymidine DMT protected intermediate; 2-S-dT DMT Toluoyl; 2-Thio-dT Phosphoramidite precursor; 5'-O-DMT-2-thiothymidine, N3,O4-di-(p-toluoyl)
EINECS	Contact for details

Quality Control

Our production of this high-purity nucleoside intermediate adheres to stringent quality protocols to ensure batch-to-batch consistency and reliability for critical synthetic workflows. Certificates of Analysis (COA) are provided with each shipment, detailing key parameters such as purity (by HPLC), identity confirmation (by NMR and MS), and moisture content. We support compliance with relevant guidelines for materials intended for use in pharmaceutical research under a quality management system.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive); containers should be sealed under an inert atmosphere (argon or nitrogen) after opening and allowed to equilibrate to room temperature before use to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, DCM, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.