Description

Uridine, 5-o-[Bis(4-Methoxyphenyl)Phenylmethyl]-2-Deoxy-2-[[(9H-Fluoren-9-Ylmethoxy)Carbonyl]Amino]-, 3-[2-Cyanoethyl Bis(1-Methylethyl)Phosphoramidite] (9Ci) is a highly specialized phosphoramidite monomer, a critical building block in the automated solid-phase synthesis of oligonucleotides. This compound is essential for researchers and manufacturers requiring precise control over nucleic acid sequence and modification. It is primarily used in the development of therapeutic oligonucleotides, diagnostic probes, and advanced research tools within the pharmaceutical and biotechnology sectors.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key phosphoramidite monomer for the automated synthesis of DNA and RNA strands.
• Therapeutic Oligonucleotide Manufacturing: Production of antisense oligonucleotides, siRNA, and aptamers for drug development.
• Diagnostic Probe Development: Synthesis of labeled probes for PCR, microarrays, and in-situ hybridization.
• Research-Grade Oligo Synthesis: For academic and industrial R&D requiring custom, high-purity oligonucleotides.
• Synthesis of Modified Nucleosides: Enabling the incorporation of specific, protected nucleoside analogs into oligonucleotide chains.
• Scale-Up GMP Production: Suitable for the cGMP-compliant manufacturing of clinical trial and commercial oligonucleotide APIs.

Basic Information

Item	Detail
Product Name	Uridine, 5-o-[Bis(4-Methoxyphenyl)Phenylmethyl]-2-Deoxy-2-[[(9H-Fluoren-9-Ylmethoxy)Carbonyl]Amino]-, 3-[2-Cyanoethyl Bis(1-Methylethyl)Phosphoramidite] (9Ci)
CAS No.	155098-83-2
Molecular Formula	C55H59N4O9P
Molecular Weight	927.06 g/mol
Synonyms	5'-O-DMT-2'-deoxy-2'-Fmoc-amino-uridine-3'-CE phosphoramidite; 2'-Deoxy-2'-Fmoc-amino-5'-O-DMT-uridine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite; 2'-Amino-2'-deoxy-5'-O-(4,4'-dimethoxytrityl)uridine-3'-(2-cyanoethyl N,N-diisopropylphosphoramidite), N-Fmoc protected; Uridine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2'-deoxy-2'-[[(9H-fluoren-9-ylmethoxy)carbonyl]amino]-, 3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite]; 2'-Fmoc-amino-2'-deoxy-5'-O-DMT-uridine CE phosphoramidite
EINECS	Contact for details

Quality Control

Our phosphoramidite monomers are manufactured under strict quality systems to ensure high purity and batch-to-batch consistency, critical for successful oligonucleotide synthesis. Each lot undergoes comprehensive analysis including HPLC purity assessment, NMR confirmation of structure, and 31P NMR for phosphorous integrity. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting all critical quality attributes.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.5%
Solubility	Clear, colorless solution in anhydrous acetonitrile
Coupling Efficiency Test	Pass

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.