Description

5-Formyl-2'',3''-o-(1-Methylethylidene)-Cytidine is a key protected nucleoside derivative, specifically a cytidine analog with formyl and isopropylidene protecting groups. This compound is essential for advanced oligonucleotide synthesis, enabling precise chemical modifications while protecting sensitive functional groups during complex reactions. It is primarily required by pharmaceutical R&D teams and fine chemical manufacturers engaged in the development of novel antiviral agents, antisense oligonucleotides, and other nucleotide-based therapeutics.

Application

• Pharmaceutical Intermediate: A critical building block in the synthesis of modified nucleosides and nucleotides for antiviral and anticancer drug candidates.
• Oligonucleotide Synthesis: Used in solid-phase and solution-phase synthesis to introduce specific cytidine modifications into DNA/RNA strands for research and therapeutic applications.
• Pro-drug Development: Serves as a precursor for creating masked or protected forms of bioactive nucleosides to improve stability and bioavailability.
• Biochemical Research: A valuable tool for studying nucleic acid structure, function, and enzyme interactions in academic and industrial laboratories.
• Antisense Technology: Employed in the manufacture of antisense oligonucleotides designed for targeted gene silencing.
• Labeled Compound Synthesis: Acts as a starting material for synthesizing radiolabeled or fluorescently tagged nucleosides for diagnostic and tracking studies.

Basic Information

Product Name	5-Formyl-2'',3''-o-(1-Methylethylidene)-Cytidine
CAS No.	153535-36-5
Molecular Formula	C14H19N3O6
Molecular Weight	325.32 g/mol
Synonyms	5-Formyl-2',3'-O-isopropylidenecytidine; 5-Formyl-2',3'-O-(1-methylethylidene)cytidine; 2',3'-O-Isopropylidene-5-formylcytidine; Cytidine, 5-formyl-2',3'-O-(1-methylethylidene)-; Protected 5-Formylcytidine; N4-Amino-1-((2R,3R,4R,5R)-3,4-dihydroxy-5-((hydroxymethyl)tetrahydrofuran-2-yl)-2-oxo-1,2-dihydropyrimidine-5-carbaldehyde 2',3'-acetonide
EINECS	Contact for details

Quality Control

Our 5-Formyl-2'',3''-o-(1-Methylethylidene)-Cytidine is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and IR for structural confirmation, and tests for residual solvents and moisture content. We provide a detailed Certificate of Analysis (COA) with every shipment to ensure full traceability and compliance with your research or cGMP-grade specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive); therefore, the container must be kept tightly sealed in a dry environment to prevent degradation. For long-term storage, consider using desiccants.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.