Description

5'-O-(Dimethoxytrityl)-5-[N-(2-(Trifluoroacetamido)Ethyl)-3-(E)-Acrylamido]-2'-Deoxyuridine is a sophisticated, high-purity nucleoside derivative engineered for advanced oligonucleotide synthesis. This compound matters as a critical building block for introducing specific, reactive functionalities into synthetic DNA strands, enabling applications in diagnostics, targeted therapeutics, and genetic research. It is primarily needed by pharmaceutical R&D teams, biotechnology companies, and specialized CROs/CMOs developing oligonucleotide-based drugs, probes, and diagnostic assays.

Application

• Oligonucleotide Synthesis: A key phosphoramidite precursor for the solid-phase synthesis of modified DNA and RNA oligonucleotides.
• Therapeutic Development: Incorporation into antisense oligonucleotides (ASOs), siRNA, and aptamers for targeted gene silencing and therapeutic applications.
• Diagnostic Probe Design: Used to create labeled or functionalized DNA probes for fluorescence in situ hybridization (FISH), PCR, and microarray technologies.
• Bioconjugation: The reactive acrylamido group serves as a handle for post-synthetic modification and conjugation with proteins, peptides, or other biomolecules.
• Chemical Biology Research: A tool compound for studying nucleic acid interactions, enzyme mechanisms, and developing novel bioassays.
• Next-Generation Sequencing (NGS): Potential use in the development of novel sequencing-by-synthesis (SBS) chemistries or specialized library preparation reagents.

Basic Information

Product Name	5'-O-(Dimethoxytrityl)-5-[N-(2-(Trifluoroacetamido)Ethyl)-3-(E)-Acrylamido]-2'-Deoxyuridine
CAS No.	153512-23-3
Molecular Formula	C39H38F3N5O9
Molecular Weight	777.74 g/mol
Synonyms	5'-O-DMT-5-[N-(2-(TFA-amino)ethyl)-3-acrylamido]-2'-deoxyuridine; DMT-dU-AA-TFA Amidite Precursor; 5-(3-Acrylamidopropyl)-2'-deoxyuridine DMT derivative; 5-[N-[2-[(Trifluoroacetyl)amino]ethyl]-3-(2-propenamido)]-5'-O-(4,4'-dimethoxytrityl)-2'-deoxyuridine; 5-(3-Acrylamidopropyl)deoxyuridine 5'-O-(4,4'-dimethoxytrityl) ether; 153512-23-3; dU-AA Amidite Synthon
EINECS	Contact for details

Quality Control

Our production of this high-value nucleoside derivative adheres to stringent quality protocols suitable for pharmaceutical research and development. Every batch is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry to ensure structural integrity and purity. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing purity, identity, and impurity profiles. Our quality management system supports compliance with cGMP principles for advanced intermediates, ensuring traceability and consistency for critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to equilibrate to room temperature before opening to prevent moisture condensation. For long-term stability, store as recommended and avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with reference structure
Purity (HPLC)	≥ 95.0% (Area %)
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.