Description

n-Acetyl-5'-o-(4,4-Dimethoxytrityl)-2'-o-[(tert-Butyl)Dimethylsilyl]-6'-o-Methylguanosine-3'-(2-Cyanoethyl-N,n-Diisopropyl)Phosphoramidite is a highly specialized, protected nucleoside phosphoramidite monomer, a critical building block in the automated solid-phase synthesis of oligonucleotides. This compound is engineered for precision and efficiency, enabling the controlled incorporation of modified guanosine residues into synthetic DNA and RNA strands. It is essential for researchers and manufacturers in the fields of therapeutic oligonucleotide development, diagnostic probe synthesis, and advanced molecular biology research, where sequence-specific functionality is paramount.

Application

• Solid-Phase Oligonucleotide Synthesis: Primary monomer for the automated synthesis of DNA/RNA sequences containing site-specific 6'-O-methylguanosine modifications.
• Antisense Oligonucleotide (ASO) Manufacturing: Key raw material for producing therapeutic ASOs with enhanced nuclease resistance and binding affinity.
• siRNA and miRNA Synthesis: Used in the production of small interfering RNA (siRNA) and microRNA (miRNA) for gene silencing research and therapeutics.
• Diagnostic Probe Development: Enables the creation of labeled probes and primers for PCR, sequencing, and microarray applications.
• Aptamer Production: Facilitates the synthesis of chemically modified aptamers for targeted drug delivery and biosensing.
• Basic Research: Fundamental reagent for academic and industrial research exploring nucleic acid structure, function, and chemical biology.

Basic Information

Product Name	n-Acetyl-5'-o-(4,4-Dimethoxytrityl)-2'-o-[(tert-Butyl)Dimethylsilyl]-6'-o-Methylguanosine-3'-(2-Cyanoethyl-N,n-Diisopropyl)Phosphoramidite
CAS No.	153248-58-9
Molecular Formula	C58H76N7O9PSi
Molecular Weight	1082.32 g/mol
Synonyms	6'-O-Methyl-2'-O-TBDMS-5'-O-DMT-N2-Acetyl-Guanosine-3'-CE Phosphoramidite; 6'-O-Me-G Phosphoramidite (2'-O-TBDMS, DMT, N-Ac); 2'-O-[(tert-Butyl)dimethylsilyl]-6'-O-methyl-5'-O-(4,4-dimethoxytrityl)-N2-acetyl-guanosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; Guanosine, N2-acetyl-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2'-O-[[(1,1-dimethylethyl)dimethylsilyl]-6'-O-methyl-, 3'-[2-cyanoethyl (diisopropylamino)phosphoramidite]; DMTr-6'-O-Me-G(N-Ac)-2'-O-TBDMS-3'-CE Phosphoramidite
EINECS	Contact for details

Quality Control

Every batch of our nucleoside phosphoramidites is produced and tested under strict quality management systems. We employ advanced analytical techniques, including HPLC, NMR, and mass spectrometry, to ensure high chemical purity and chiral integrity, which are critical for successful oligonucleotide synthesis. A comprehensive Certificate of Analysis (COA) detailing purity, moisture content, and solvent residues is provided with each shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This compound is hygroscopic (moisture-sensitive) and easily oxidized; prolonged exposure to air, light, or ambient humidity will lead to decomposition and reduced coupling efficiency. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Moisture Content (KF)	≤ 1.0%
Solvent Residues (GC)	Meet ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.