Description

4'-Methyl-5-Methyluridine is a modified nucleoside derivative of uridine, featuring methyl groups at both the 4' and 5 positions of the ribose and pyrimidine rings, respectively. This structural modification is critical for its role as a key intermediate and building block in the synthesis of novel oligonucleotides and therapeutic nucleic acids. It is primarily utilized by research institutions and pharmaceutical companies engaged in the development of advanced RNA-based therapeutics, antiviral agents, and molecular biology tools. The compound's defined structure enables precise control over the properties and stability of the resulting biopolymers.

Application

• Pharmaceutical Intermediate: A crucial building block for the synthesis of modified nucleoside analogs used in antiviral and anticancer drug discovery.
• Oligonucleotide Synthesis: Used in solid-phase synthesis to incorporate specific modifications into RNA strands, enhancing their stability, binding affinity, and therapeutic potential.
• Biochemical Research: Serves as a substrate or inhibitor in enzymatic studies to understand RNA metabolism, repair, and processing mechanisms.
• Antisense & RNAi Technology: Incorporated into antisense oligonucleotides or siRNA to improve nuclease resistance and pharmacokinetic profiles.
• Diagnostic Probe Development: Used in the creation of labeled probes for detecting specific RNA sequences in diagnostic assays.
• mRNA Vaccine & Therapeutic Research: Investigated for potential use in modifying mRNA constructs to modulate immunogenicity and translation efficiency.

Basic Information

Product Name	4'-Methyl-5-Methyluridine
CAS No.	152540-75-5
Molecular Formula	C11H16N2O6
Molecular Weight	272.25 g/mol
Synonyms	5-Methyl-4'-methyluridine; 4'-C-Methyl-5-methyluridine; 1-((2R,3R,4S,5R)-3,4-Dihydroxy-4-methyl-5-(hydroxymethyl)oxolan-2-yl)-5-methylpyrimidine-2,4-dione; 4'-Methyl-5-methyl-Uridine; 5-Methyl-4'-C-methyluridine; Modified Uridine Derivative; Nucleoside Intermediate
EINECS	Contact for details

Quality Control

Our 4'-Methyl-5-Methyluridine is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and tests for residual solvents and moisture content. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with research-grade specifications. Custom synthesis and purification to meet specific project requirements (e.g., >98% or >99% HPLC purity) are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic nature, the container should be kept in a desiccated environment to prevent moisture uptake, which may affect stability and handling. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 2.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.