Description

1-(2',3'-Epithio-2',3'-Dideoxy-β-D-Lyxofuranosyl)Thymine 5'-Triphosphate is a specialized, high-purity nucleotide analog designed for advanced biochemical and pharmaceutical research. This compound is critical for applications requiring precise molecular probes or chain-terminating substrates due to its modified sugar-phosphate backbone. It is primarily utilized by research institutions and pharmaceutical developers in the fields of molecular biology, enzymology, and next-generation sequencing (NGS) technology development. The product, identified by CAS No. 152158-09-3, is supplied with stringent quality control to ensure batch-to-batch consistency for sensitive experimental workflows.

Application

• Next-Generation Sequencing (NGS): Acts as a specialized substrate or terminator in novel sequencing-by-synthesis platforms.
• Enzymatic Studies: Used as a probe to investigate the activity and specificity of DNA polymerases and reverse transcriptases.
• Antiviral Drug Discovery: Serves as a potential nucleotide analog precursor in the research and development of antiviral therapeutics.
• Molecular Biology Research: Employed in the study of DNA replication, repair mechanisms, and nucleic acid metabolism.
• Diagnostic Assay Development: Integrated into the design of sensitive probes for pathogen detection or genetic mutation analysis.
• Biochemical Synthesis: Used in the enzymatic synthesis of modified oligonucleotides with specific functional properties.

Basic Information

Product Name	1-(2',3'-Epithio-2',3'-Dideoxy-β-D-Lyxofuranosyl)Thymine 5'-Triphosphate
CAS No.	152158-09-3
Molecular Formula	C15H22N3O13P3S
Molecular Weight	577.32 g/mol
Synonyms	2',3'-Epithio-2',3'-dideoxylyxofuranosyl thymine 5'-triphosphate; Thymine 5'-triphosphate, 1-(2,3-epithio-2,3-dideoxy-β-D-lyxofuranosyl)-; SET-dTTP; Epithio-dTTP; Modified Nucleotide Triphosphate; Chain-terminating nucleotide analog; 1-[(2R,3R,4R,5R)-5-(Hydroxyphosphinatooxyphosphoryl)oxymethyl-3,4-epithio-3,4-dihydro-2-furanyl]-5-methyl-2,4(1H,3H)-pyrimidinedione trianion
EINECS	Contact for details

Quality Control

Our production of this nucleotide analog adheres to strict quality management protocols to ensure it meets the exacting standards required for life science research. Each batch is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity, purity, and absence of critical impurities like nucleobase contaminants or unreacted precursors. A comprehensive Certificate of Analysis (COA) detailing purity, assay results, and impurity profiles is provided with every shipment to support your regulatory and research documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dedicated, non-frost-free freezer. The product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions. Allow the vial to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay (UV)	≥ 90% (anhydrous basis)
Water Content (KF)	≤ 3.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.