Description

2'-(n-Methylanthraniloyl)Adenosine Triphosphate, Sodium Salt is a fluorescently labeled ATP analog, a crucial tool compound for real-time monitoring of ATP-dependent enzymatic activity. Its core value lies in providing a sensitive, non-radioactive method for studying kinases, ATPases, and other nucleotide-binding proteins in high-throughput screening and mechanistic biochemistry. This product is essential for researchers and developers in pharmaceutical discovery, biochemical assay development, and life science research who require precise, reliable probes for energy transfer and binding studies.

Application

• Fluorescent Probe for Kinase Activity Assays: Used in homogeneous time-resolved fluorescence (HTRF) and fluorescence polarization (FP) assays to measure kinase activity and inhibition.
• ATPase and Motor Protein Studies: Serves as a substrate to monitor the hydrolysis cycle of ATPases, including myosin, kinesin, and helicases, in real-time.
• High-Throughput Screening (HTS): A key reagent in drug discovery campaigns targeting ATP-binding sites for the identification of novel enzyme inhibitors.
• Biophysical Binding Studies: Employed in fluorescence anisotropy or Förster resonance energy transfer (FRET) experiments to quantify protein-ligand interactions and conformational changes.
• Diagnostic Assay Development: Used in the development of clinical and research diagnostic kits for monitoring enzymatic pathways related to cellular energy metabolism.
• Academic Research: Fundamental tool for investigating the mechanisms of signal transduction, cell cycle regulation, and energy metabolism in academic laboratories.

Basic Information

Product Name	2'-(n-Methylanthraniloyl)Adenosine Triphosphate, Sodium Salt
CAS No.	151481-86-6
Molecular Formula	C23H26N6O15P3Nax (x≈3)
Molecular Weight	~829.4 g/mol (Free acid, anhydrous basis)
Synonyms	MANT-ATP, Sodium Salt; 2'-(N-Methylanthraniloyl)-ATP; 2'-O-(N-Methylanthraniloyl)adenosine 5'-triphosphate; MANT-ATP, Trisodium Salt; Fluorescent ATP analog; 2'-Deoxy-2'-(N-methylanthraniloylamino)adenosine triphosphate; 2'-MANT-ATP; ATP, 2'-(N-methylanthraniloyl)-, sodium salt
EINECS	Contact for details

Quality Control

Our 2'-(n-Methylanthraniloyl)Adenosine Triphosphate, Sodium Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and performance in sensitive assays. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional performance data. We adhere to rigorous analytical standards to meet the demands of research and screening applications. Certificates of Analysis (COA) are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep desiccated. Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange solid or lyophilized powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (UV/VIS)	Spectrum characteristic of MANT chromophore
Purity (HPLC)	≥95% (Area percent at 254 nm / 360 nm)
Assay (Enzymatic)	Functional activity confirmed vs. standard ATP
Water Content (KF)	≤5.0%
Solubility	Soluble in aqueous buffer, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.