Description

2'-Deoxy-5'-o-Dmt-N2-Isobutyryl-A-Guanosine is a high-purity, protected nucleoside phosphoramidite precursor, essential for the solid-phase synthesis of oligonucleotides. This compound is critical for ensuring high coupling efficiency and sequence fidelity in the production of DNA and RNA strands. It is primarily required by manufacturers and research institutions in the pharmaceutical, biotechnology, and diagnostic sectors for applications in antisense therapy, PCR primers, and genetic research.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite building block in automated solid-phase DNA/RNA synthesizers.
• Therapeutic Oligonucleotide Production: For the manufacture of antisense oligonucleotides, siRNA, and aptamers for drug development.
• Research & Diagnostic Reagents: Used in synthesizing primers, probes, and standards for PCR, qPCR, and sequencing.
• Genomic Studies: Enables the custom synthesis of gene fragments and modified oligonucleotides for functional genomics.
• Bioconjugation: Serves as a starting material for creating labeled nucleotides for detection and imaging.
• CRISPR/Cas9 System Components: For the synthesis of guide RNAs (gRNAs) and other synthetic nucleic acid tools.

Basic Information

Product Name	2'-Deoxy-5'-o-Dmt-N2-Isobutyryl-A-Guanosine
CAS No.	149099-40-1
Molecular Formula	C41H47N5O8
Molecular Weight	737.85 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N2-isobutyryl-2'-deoxyguanosine; DMT-dG(ib)-CE Phosphoramidite precursor; N2-Isobutyryl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxyguanosine; 2'-Deoxy-5'-O-DMT-N2-isobutyrylguanosine; Protected dG Phosphoramidite; Guanosine, 2'-deoxy-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-N2-(2-methyl-1-oxopropyl)-; dG(ib) DMT
EINECS	Contact for details

Quality Control

Our 2'-Deoxy-5'-o-Dmt-N2-Isobutyryl-A-Guanosine is manufactured under strict quality systems to meet the exacting standards of oligonucleotide synthesis. Each batch is subjected to rigorous analytical testing, including HPLC, NMR, and mass spectrometry, to ensure high purity and identity. Certificates of Analysis (COA) detailing all specifications are provided to guarantee batch-to-batch consistency and support your regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic nature, the product should be handled in a dry environment and sealed under an inert atmosphere (argon or nitrogen) after opening for long-term stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, DMF, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.