Description

4-Azidophenacyl Lithocholate is a specialized lithocholic acid derivative functionalized with a photoreactive azidophenacyl group. This compound is engineered as a critical chemical probe for studying protein-lipid interactions and membrane dynamics through photoaffinity labeling techniques. It is primarily utilized by research scientists and pharmaceutical developers in the fields of biochemistry, medicinal chemistry, and metabolic disease research.

Application

• Photoaffinity Labeling Probe: Used to covalently cross-link and identify bile acid receptors and transporters in biological membranes.
• Biochemical Research: Serves as a key intermediate in the synthesis of probes for studying the Farnesoid X Receptor (FXR) and other nuclear receptors.
• Medicinal Chemistry: Employed in the development and screening of novel therapeutics targeting bile acid pathways and cholesterol metabolism.
• Proteomics Studies: Facilitates the isolation and characterization of proteins that interact with lithocholic acid and its derivatives.
• Chemical Biology Tool: Enables the mapping of binding sites and interaction networks of steroidal compounds within complex cellular systems.

Basic Information

Product Name	4-Azidophenacyl Lithocholate
CAS No.	149021-83-0
Molecular Formula	C31H41N3O4
Molecular Weight	519.68 g/mol
Synonyms	Lithocholic acid 3-(4-azidophenacyl) ester; 3α-Hydroxy-5β-cholan-24-oic acid 3-(4-azidophenacyl) ester; 4-Azidophenacyl 3α-hydroxy-5β-cholan-24-oate; N-(4-Azidophenacyl)lithocholamide (common misnomer); APL; Photoactivatable lithocholate derivative; Bile acid photoaffinity label
EINECS	Contact for details

Quality Control

Our 4-Azidophenacyl Lithocholate is produced under strict quality management protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label or COA. This material is hygroscopic (moisture-sensitive) and light-sensitive; therefore, containers must be kept sealed under an inert atmosphere and opened only in a controlled environment to minimize degradation.

Specification

Item	Specification
Appearance	Off-white to light yellow powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.