Description

5-Bromo-4-Chloro-3-Indolyltetraphospho-5'-Adenosine is a specialized nucleotide analog used as a critical reagent in advanced biochemical research and diagnostic development. Its primary value lies in its role as a chromogenic substrate for detecting specific enzymatic activities, particularly phosphatases, enabling precise visualization in assays. This compound is essential for researchers and manufacturers in the fields of molecular biology, diagnostic kit production, and pharmaceutical screening who require high-purity, reliable biochemical tools.

Application

• Enzyme Substrate for Phosphatases: Acts as a chromogenic substrate in histochemical and biochemical assays for detecting alkaline phosphatase and related enzyme activity.
• Molecular Biology Research: Used in nucleic acid detection techniques, including blotting and in situ hybridization protocols, for colorimetric signal generation.
• Diagnostic Kit Manufacturing: A key component in the formulation of clinical diagnostic kits for various disease markers, ensuring high sensitivity and specificity.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) assays to identify and characterize potential drug candidates that modulate phosphatase pathways.
• Cell Biology & Immunology: Facilitates the study of cellular signaling pathways and immune cell functions through colorimetric detection methods.
• Biochemical Reagent Production: Serves as a raw material for producing specialized reagent mixes and buffers used in life science laboratories.

Basic Information

Product Name	5-Bromo-4-Chloro-3-Indolyltetraphospho-5'-Adenosine
CAS No.	148778-60-3
Molecular Formula	C19H22BrClN6O15P4
Molecular Weight	827.62 g/mol
Synonyms	5-Bromo-4-chloro-3-indolyl tetraphospho-5'-adenosine; BCIP-tetraphosphate-adenosine; 5-Bromo-4-chloro-1H-indol-3-yl tetraphospho-5'-adenosine; Adenosine 5'-(tetrahydrogen tetraphosphate), 5'-5''-ester with 5-bromo-4-chloro-1H-indol-3-ol; BCIPTPA; X-Phosphate-adenosine tetraphosphate; Chromogenic phosphatase substrate analog; 5-Br-4-Cl-3-indolyl-P4-adenosine
EINECS	Contact for details

Quality Control

Our 5-Bromo-4-Chloro-3-Indolyltetraphospho-5'-Adenosine is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to guarantee traceability and consistency.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store desiccated under inert atmosphere. Allow the container to reach room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 3.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.