Description

Dmt-2′Fluoro-Du Phosphoramidite is a specialized, high-purity phosphoramidite monomer used in the automated solid-phase synthesis of oligonucleotides. This compound is critical for introducing a 2'-fluoro modification into nucleic acid strands, a key step in creating research-grade and therapeutic oligonucleotides with enhanced stability and binding affinity. It is an essential raw material for biotechnology, pharmaceutical R&D, and diagnostic kit manufacturers focused on antisense, siRNA, and aptamer development.

Application

• Therapeutic Oligonucleotide Synthesis: Key building block for manufacturing antisense oligonucleotides (ASOs) and small interfering RNAs (siRNAs) with 2'-fluoro sugar modifications to improve nuclease resistance and pharmacokinetic properties.
• Research & Diagnostic Probes: Used in the synthesis of fluorescently labeled or modified DNA/RNA probes for advanced molecular biology research, FISH (Fluorescence In Situ Hybridization), and PCR applications requiring high specificity.
• Aptamer Development: Enables the creation of stable nucleic acid aptamers for biosensing, diagnostic assays, and targeted therapeutic delivery systems.
• CRISPR Guide RNA Modification: Employed to synthesize chemically modified guide RNAs (gRNAs) to enhance the stability and efficiency of CRISPR-Cas gene editing systems.
• Pharmaceutical Intermediates: Serves as a critical intermediate in the multi-step production of active pharmaceutical ingredients (APIs) for novel nucleic acid-based drugs.

Basic Information

Product Name	Dmt-2′Fluoro-Du Phosphoramidite
CAS No.	146954-75-8
Molecular Formula	C41H49FN3O8P
Molecular Weight	785.82 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-fluoro-2'-deoxyuridine 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 2'-F-dU Phosphoramidite; 2'-Fluoro-2'-deoxyuridine Phosphoramidite; DMT-2'F-dU-CE Phosphoramidite; 2'-F-dU-CE Phosphoramidite; 5'-DMT-2'-fluoro-2'-deoxyuridine-3'-CE phosphoramidite; 2'-Fluoro-deoxyuridine Phosphoramidite
EINECS	Contact for details

Quality Control

Every batch of Dmt-2′Fluoro-Du Phosphoramidite is manufactured under strict quality management systems and undergoes comprehensive analytical testing to ensure it meets the high-purity standards required for oligonucleotide synthesis. We provide full traceability and detailed Certificates of Analysis (COA) with each shipment, documenting purity (typically ≥98% by HPLC), identity, and moisture content. Our quality protocols are designed to support GMP-grade production for therapeutic applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, freezer. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to air, moisture, or ambient light will degrade product quality. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Freely soluble in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.