Description

2'-Deoxy-5'-o-Dmt-2'-Fluorouridine is a high-purity, protected nucleoside phosphoramidite building block, essential for the solid-phase synthesis of oligonucleotides. This compound matters for its critical role in enabling the precise and efficient incorporation of modified bases into synthetic DNA and RNA strands, which is fundamental for advanced research and therapeutic development. It is primarily needed by pharmaceutical R&D teams, biotechnology companies, and academic laboratories engaged in antisense therapy, siRNA development, diagnostic probe synthesis, and other nucleic acid-based applications.

Application

• Oligonucleotide Synthesis: A key phosphoramidite monomer for the automated solid-phase synthesis of DNA and RNA strands.
• Antisense Oligonucleotide (ASO) Development: Used in the research and production of therapeutic ASOs targeting specific mRNA sequences.
• siRNA and miRNA Synthesis: Critical for constructing short interfering RNA (siRNA) and microRNA (miRNA) molecules for gene silencing studies and therapeutics.
• Diagnostic Probe Manufacture: Employed in creating labeled probes for fluorescence in situ hybridization (FISH), PCR, and other molecular diagnostics.
• Structural Biology Studies: Facilitates the synthesis of modified oligonucleotides for investigating nucleic acid structure, stability, and function.
• Aptamer Development: Used in the generation of synthetic aptamers for therapeutic and sensor applications.
• Pharmaceutical Intermediate: Serves as a crucial intermediate in the synthesis of novel nucleoside-based antiviral or anticancer drug candidates.

Basic Information

Product Name	2'-Deoxy-5'-o-Dmt-2'-Fluorouridine
CAS No.	146954-74-7
Molecular Formula	C30H29FN2O7
Molecular Weight	548.57 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-deoxy-2'-fluorouridine; 2'-Deoxy-2'-fluoro-5'-O-DMT-uridine; 2'-Fluoro-2'-deoxy-5'-O-(4,4'-dimethoxytrityl)uridine; 2'-F-dU(DMT); 1-[(2R,3R,4R,5R)-5-[[Bis(4-methoxyphenyl)-phenylmethoxy]methyl]-3-fluoro-4-hydroxyoxolan-2-yl]pyrimidine-2,4-dione; DMT-2'F-dU; 2'-Fluoro-5'-O-DMT-2'-deoxyuridine; Protected 2'-Fluorouridine Phosphoramidite Precursor
EINECS	Contact for details

Quality Control

Our 2'-Deoxy-5'-o-Dmt-2'-Fluorouridine is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity, suitable for sensitive oligonucleotide synthesis. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing specific results for assay, related substances, and moisture content are available upon request. We adhere to cGMP guidelines for the production of pharmaceutical intermediates.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) in a dry, freezer. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solvent Residues (GC)	Meets ICH limits

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.