Description

5'-O-(4,4-Dimethoxytrityl)-3'-Deoxy-5-Methyluridine is a protected nucleoside derivative, a critical building block in the synthesis of oligonucleotides and modified nucleic acids. Its primary value lies in enabling the precise, automated synthesis of DNA/RNA sequences with specific modifications for research and therapeutic applications. This high-purity intermediate is essential for pharmaceutical R&D, biotechnology, and academic laboratories focused on antisense oligonucleotides, siRNA, aptamers, and other advanced nucleic acid-based technologies.

Application

• Solid-Phase Oligonucleotide Synthesis (SPOS): As a phosphoramidite precursor for the automated synthesis of oligonucleotides containing modified nucleosides.
• Antisense Oligonucleotide (ASO) Development: A key raw material for creating therapeutic oligonucleotides designed to target specific RNA sequences.
• siRNA and miRNA Research: Used in the preparation of short interfering RNA (siRNA) and microRNA (miRNA) analogs for gene silencing studies.
• Aptamer Production: Enables the synthesis of chemically modified DNA/RNA libraries for SELEX processes to develop high-affinity aptamers.
• Diagnostic Probe Synthesis: Incorporated into labeled nucleic acid probes for diagnostic assays and hybridization techniques.
• Pharmaceutical Intermediate: Serves as a vital intermediate in the GMP-compliant manufacturing of active pharmaceutical ingredients (APIs) for nucleic acid therapeutics.

Basic Information

Product Name	5'-O-(4,4-Dimethoxytrityl)-3'-Deoxy-5-Methyluridine
CAS No.	143653-61-6
Molecular Formula	C32H34N2O8
Molecular Weight	574.62 g/mol
Synonyms	5'-O-DMT-3'-deoxy-5-methyluridine; 3'-Deoxy-5-methyluridine 5'-(4,4'-dimethoxytrityl) ether; DMT-3'-deoxy-5-methyluridine; 1-[(2R,5S)-5-({bis[(4-methoxyphenyl)(phenyl)methoxy]methyl}oxolan-2-yl]-5-methylpyrimidine-2,4-dione; 5-Methyl-2'-deoxyuridine 5'-O-DMT derivative; Nucleoside phosphoramidite precursor; Protected 3'-deoxy-5-methyluridine
EINECS	Contact for details

Quality Control

Every batch of 5'-O-(4,4-Dimethoxytrityl)-3'-Deoxy-5-Methyluridine is manufactured and tested under strict quality systems to ensure it meets the high-purity standards required for sensitive oligonucleotide synthesis. Our quality commitment includes comprehensive analytical characterization by HPLC, NMR, and mass spectrometry. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are provided with each shipment to support your quality and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This compound is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.