Description

N6-Benzoyl-5'-o-(Dimethoxytrityl)-8-Hydroxy-2'-Deoxyadenosine CAS NO 142979-41-7 is a high-purity, protected nucleoside derivative specifically engineered for oligonucleotide synthesis. This compound serves as a critical phosphoramidite building block, enabling the precise and efficient assembly of DNA sequences for research and therapeutic applications. It is an essential raw material for pharmaceutical R&D, diagnostic assay development, and advanced life science research requiring modified nucleic acids.

Application

• Oligonucleotide Synthesis: A key phosphoramidite monomer for the solid-phase synthesis of DNA strands, particularly those requiring modified bases.
• Antisense Drug Development: Used in the research and production of antisense oligonucleotides (ASOs) for therapeutic applications targeting genetic diseases.
• PCR Primer & Probe Manufacture: Enables the creation of specialized primers and fluorescent probes with high specificity for diagnostic and research qPCR assays.
• siRNA and miRNA Synthesis: A crucial component in the production of short interfering RNA (siRNA) and microRNA (miRNA) for gene silencing studies and RNAi therapeutics.
• DNA Microarray Fabrication: Employed in the synthesis of high-density DNA probes immobilized on chips for genomic analysis and screening.
• Academic & Industrial Research: Supports fundamental biochemical research involving nucleic acid structure, function, and enzyme interactions.
• Next-Generation Sequencing (NGS): Used in the development of specialized adapters and library preparation reagents for advanced sequencing platforms.
• Aptamer Development: Facilitates the synthesis of chemically modified DNA libraries for SELEX processes to generate high-affinity aptamers.

Basic Information

Product Name	N6-Benzoyl-5'-o-(Dimethoxytrityl)-8-Hydroxy-2'-Deoxyadenosine
CAS No.	142979-41-7
Molecular Formula	C44H39N5O7
Molecular Weight	749.82 g/mol
Synonyms	8-Hydroxy-2'-deoxyadenosine, N6-benzoyl-5'-O-(4,4'-dimethoxytrityl)-; 5'-O-DMT-N6-benzoyl-8-oxo-2'-deoxyadenosine; 8-Oxo-dA(Bz) Phosphoramidite precursor; N6-Bz-8-OH-dA(DMT); Protected 8-hydroxy-2'-deoxyadenosine; DMT-8-OH-dA(Bz); 2'-Deoxy-8-hydroxyadenosine derivative
EINECS	Contact for details

Quality Control

Our N6-Benzoyl-5'-o-(Dimethoxytrityl)-8-Hydroxy-2'-Deoxyadenosine is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for sensitive oligonucleotide synthesis. All materials undergo rigorous analytical testing, including HPLC, NMR, and mass spectrometry, to confirm identity, purity, and the absence of critical impurities. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with each shipment to support your quality assurance and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation. For long-term stability, store under an inert atmosphere such as argon or nitrogen.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, DMF, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.