Description

N4-Benzoyl-2'-Deoxy-5'-o-(4,4'-Dimethoxytrityl)-2',2'-Diflurocytidine is a sophisticated, high-purity nucleoside phosphoramidite derivative, a critical building block in the synthesis of oligonucleotides. This compound is essential for enabling the precise and efficient construction of therapeutic nucleic acids, including antisense oligonucleotides and siRNA. It is primarily utilized by pharmaceutical R&D teams, biotechnology companies, and CROs specializing in the development of advanced genetic medicines and molecular diagnostics.

Application

• Solid-Phase Oligonucleotide Synthesis: A key phosphoramidite monomer for the automated synthesis of DNA and RNA analogs, particularly those containing 2',2'-difluorocytidine modifications.
• Therapeutic Oligonucleotide Manufacturing: Critical raw material in the GMP production of antisense drugs, siRNA therapeutics, and aptamers targeting various diseases.
• Research & Development: Used in academic and industrial labs for studying nucleic acid structure, function, and for developing novel gene-silencing or gene-editing tools.
• Diagnostic Probe Synthesis: Employed in the creation of highly specific fluorescent or labeled probes for PCR, FISH, and other molecular diagnostic assays.
• Scale-Up and Process Development: Serves as a starting material for process chemistry teams optimizing routes for large-scale oligonucleotide active pharmaceutical ingredient (API) production.

Basic Information

Item	Details
Product Name	N4-Benzoyl-2'-Deoxy-5'-o-(4,4'-Dimethoxytrityl)-2',2'-Diflurocytidine
CAS No.	142808-43-3
Molecular Formula	C43H39F2N3O7
Molecular Weight	747.79 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N4-benzoyl-2'-deoxy-2',2'-difluorocytidine; 2'-Deoxy-2',2'-difluorocytidine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-N4-benzoyl-; DMT-2'-F-2'-F-C(Bz) Phosphoramidite precursor; 2',2'-Difluoro-2'-deoxycytidine DMT-protected derivative; N4-Benzoyl-5'-O-DMT-2'-deoxy-2',2'-difluorocytidine; Gemcitabine intermediate (protected); 5'-O-DMT-N4-Bz-2'-deoxy-2',2'-difluorocytidine
EINECS	Contact for details

Quality Control

Every batch of N4-Benzoyl-2'-Deoxy-5'-o-(4,4'-Dimethoxytrityl)-2',2'-Diflurocytidine is manufactured and tested under strict quality systems to ensure it meets the exacting standards required for oligonucleotide synthesis. We provide comprehensive analytical data including HPLC purity (typically >98%), identity confirmation by NMR and mass spectrometry, and stringent control of moisture content. A detailed Certificate of Analysis (COA) is supplied with each shipment, and custom synthesis to meet specific purity or packaging requirements is available.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under an inert atmosphere (argon or nitrogen) due to its hygroscopic and moisture-sensitive nature. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solvent Residues (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.