Description

N(3)-Methyl-3'-Azido-3'-Deoxythymidine 5'-Triphosphate is a chemically modified nucleoside triphosphate analog, a critical building block for advanced biochemical research and development. Its core value lies in its ability to act as a chain terminator or a substrate for specialized polymerases, enabling precise manipulation of nucleic acid sequences. This compound is essential for researchers and developers in the fields of molecular biology, antiviral drug discovery, and next-generation sequencing technologies.

Application

• Chain Termination in DNA Sequencing: Used in Sanger sequencing and related methodologies to halt DNA strand elongation at specific modified bases.
• Antiviral Research: Serves as a potential substrate analog for studying viral reverse transcriptase inhibition, relevant to HIV and other retroviral research.
• Probe Synthesis: Enables the enzymatic incorporation of azide-labeled nucleotides for creating functionalized nucleic acid probes.
• Click Chemistry Conjugation: The azido group allows for bioorthogonal "click" reactions (e.g., with alkynes) for labeling, pull-down assays, or surface immobilization of nucleic acids.
• Enzymology Studies: A tool for investigating the substrate specificity and kinetics of DNA polymerases and other nucleic acid-processing enzymes.
• Synthetic Biology: Used in the engineering of novel genetic circuits and systems that require chemically modified genetic components.

Basic Information

Product Name	N(3)-Methyl-3'-Azido-3'-Deoxythymidine 5'-Triphosphate
CAS No.	142644-39-1
Molecular Formula	C11H16N6O13P3
Molecular Weight	548.19 g/mol
Synonyms	3'-Azido-3'-deoxy-N3-methylthymidine 5'-triphosphate; 3'-Azido-3'-deoxy-N3-methylthymidine triphosphate; 3'-Azido-N3-methylthymidine triphosphate; AZT-Methyl-TP; N3-Me-AZT-TP; 3'-Azido-2',3'-dideoxy-N3-methylthymidine 5'-triphosphate; Modified dTTP analog; Azido-modified nucleotide triphosphate
EINECS	Contact for details

Quality Control

Our N(3)-Methyl-3'-Azido-3'-Deoxythymidine 5'-Triphosphate is produced under stringent quality control protocols to ensure batch-to-batch consistency and high purity for research applications. Each lot is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. The product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation. Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, TE buffer, or DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.