Description

3'-Azidothymidine-5'-Bis(4-Nitrophenyl)Phosphate is a chemically modified nucleoside derivative, specifically a bis(4-nitrophenyl) phosphate ester of the antiviral compound AZT (Azidothymidine). This compound is a critical building block (phosphoramidite precursor) in oligonucleotide synthesis, enabling the site-specific incorporation of modified bases for advanced research and therapeutic development. It is primarily utilized by pharmaceutical R&D teams, biotech companies, and academic laboratories focused on antisense technology, siRNA, and novel nucleic acid-based drug discovery.

Application

• Oligonucleotide Synthesis: Acts as a key phosphoramidite monomer for the solid-phase synthesis of modified DNA and RNA strands.
• Antisense & siRNA Drug Development: Used to create oligonucleotides with enhanced stability and specific biological activity for therapeutic applications.
• Proteomics & Genomics Research: Serves as a specialized reagent in the study of nucleic acid-protein interactions and gene function.
• Diagnostic Probe Development: Employed in the manufacture of labeled probes for sensitive detection and diagnostic assays.
• Bioconjugation & Labeling: Facilitates the attachment of reporter molecules or other functional groups to nucleic acids.
• Academic & Industrial R&D: A vital tool for chemists and biologists exploring novel nucleotide analogs and their biochemical properties.

Basic Information

Product Name	3'-Azidothymidine-5'-Bis(4-Nitrophenyl)Phosphate
CAS No.	142629-84-3
Molecular Formula	C28H24N6O11P
Molecular Weight	651.49 g/mol
Synonyms	AZT-5'-Bis(4-Nitrophenyl) Phosphate; 3'-Azido-3'-deoxythymidine 5'-(bis(4-nitrophenyl) phosphate); Thymidine, 3'-azido-3'-deoxy-, 5'-(bis(4-nitrophenyl) phosphate); AZT Phosphoramidite Precursor; 5'-O-(Bis(4-nitrophenyl)phosphoryl)-3'-azido-3'-deoxythymidine; Nucleoside Phosphorylation Reagent; 3'-N3-Thymidine-5'-bis(p-Nitrophenyl) Phosphate
EINECS	Contact for details

Quality Control

Our 3'-Azidothymidine-5'-Bis(4-Nitrophenyl)Phosphate is produced under stringent conditions to ensure high purity and batch-to-batch consistency, suitable for sensitive research and development applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and NMR for structural confirmation. Certificates of Analysis (COA) detailing all specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label or COA. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. Keep the container sealed in a desiccator when not in use.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in anhydrous acetonitrile, DMF, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.