Description

S4-(2-Cyanoethyl)-5'-o-(Dimethoxytrityl)-4-Thiothymidine is a high-purity, chemically modified nucleoside derivative, specifically engineered as a critical building block for oligonucleotide synthesis. This compound is essential for the development of antisense oligonucleotides, siRNA, and other therapeutic nucleic acids, where its 4-thio modification and protective groups enable precise control over structure and function. It is primarily utilized by pharmaceutical R&D departments, biotechnology companies, and advanced academic research institutions focused on next-generation genetic medicines and molecular diagnostics.

Application

• Oligonucleotide Synthesis: A key phosphoramidite precursor for the solid-phase synthesis of therapeutic oligonucleotides containing 4-thiothymidine modifications.
• Antisense Drug Development: Used in the research and production of antisense oligonucleotides (ASOs) targeting specific mRNA sequences for therapeutic intervention.
• siRNA and RNAi Therapeutics: Serves as a modified nucleotide component to enhance the stability, specificity, and pharmacokinetic properties of small interfering RNA (siRNA) constructs.
• Diagnostic Probe Fabrication: Incorporated into nucleic acid probes for diagnostic assays, where the thio-modification can be used for labeling or to alter hybridization properties.
• Structural Biology Studies: Employed in research to study nucleic acid structure, function, and protein-nucleic acid interactions through site-specific modification.
• Apitamer Development: Utilized in the synthesis of modified DNA/RNA libraries for SELEX processes to generate high-affinity aptamers with improved nuclease resistance.

Basic Information

Product Name	S4-(2-Cyanoethyl)-5'-o-(Dimethoxytrityl)-4-Thiothymidine
CAS No.	142409-74-3
Molecular Formula	C34H35N3O6S
Molecular Weight	613.73 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-4-thio-2'-deoxythymidine 3'-[S-(2-Cyanoethyl)] Phosphorothioate Triester; 4-Thiothymidine Derivative; DMT-4-thio-dT-CE Phosphoramidite; 2'-Deoxy-4-thiothymidine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-, 3'-[S-(2-cyanoethyl) phosphorothioate]; 4-Thio-dT-CE Phosphoramidite; 4-ThioT Phosphoramidite; S-2-Cyanoethyl 5'-O-(4,4'-dimethoxytrityl)-4-thiothymidine-3'-O-(N,N-diisopropyl)phosphoramidite (common precursor form)
EINECS	Contact for details

Quality Control

Our S4-(2-Cyanoethyl)-5'-o-(Dimethoxytrityl)-4-Thiothymidine is manufactured under strict quality management systems to ensure the high purity and consistency required for sensitive oligonucleotide synthesis. Each batch is subjected to comprehensive analytical testing, including HPLC, NMR, and mass spectrometry, to verify identity, purity, and absence of critical impurities. Certificates of Analysis (COA) detailing all specifications are provided with every shipment. We support compliance with cGMP and ISO 9001 standards for pharmaceutical intermediates.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This material is strictly light-sensitive (protect from all light exposure) and hygroscopic (moisture-sensitive). Allow the sealed container to equilibrate to room temperature before opening to prevent moisture condensation. For long-term storage, keep under an inert atmosphere such as argon or nitrogen.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in anhydrous acetonitrile, DMF, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.