Description

5'-o-(Dimethoxytrityl)-5-Fluoro-O4-(2,4,6-Trimethylphenyl)-2'-Deoxyuridine CAS NO 141120-64-1 is a sophisticated, high-purity nucleoside derivative specifically engineered for advanced oligonucleotide synthesis. This compound serves as a critical protected phosphoramidite precursor, enabling the precise incorporation of modified bases into synthetic DNA and RNA strands. It is an essential building block for research and development in antisense therapeutics, diagnostic probes, and molecular biology, where sequence specificity and functional group integrity are paramount.

Application

• Synthesis of Antisense Oligonucleotides (ASOs): Used as a key monomer for creating therapeutic oligonucleotides targeting specific mRNA sequences.
• Diagnostic Probe Development: Essential for manufacturing fluorescently labeled or otherwise tagged DNA probes used in PCR, FISH, and microarray technologies.
• siRNA and miRNA Research: Acts as a modified nucleotide precursor in the solid-phase synthesis of short interfering RNA (siRNA) and microRNA (miRNA) for gene silencing studies.
• Aptamer Production: Employed in the chemical synthesis of DNA aptamers, which are used as molecular recognition elements in biosensors and therapeutics.
• Structural Studies of Nucleic Acids: Incorporated into oligonucleotides to study the impact of base modifications on DNA/RNA structure, stability, and protein interactions.
• Pharmaceutical Intermediate: Serves as a crucial intermediate in the development and cGMP manufacturing of nucleotide-based active pharmaceutical ingredients (APIs).

Basic Information

Product Name	5'-o-(Dimethoxytrityl)-5-Fluoro-O4-(2,4,6-Trimethylphenyl)-2'-Deoxyuridine
CAS No.	141120-64-1
Molecular Formula	C41H41FN2O7
Molecular Weight	692.78 g/mol
Synonyms	5'-O-DMT-5-Fluoro-O4-(2,4,6-Trimethylphenyl)-2'-deoxyuridine; 5-Fluoro-2'-deoxyuridine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-O4-(2,4,6-trimethylphenyl)-; DMT-FdU(Mes) Amidite Precursor; 5-Fluoro-O4-mesityl-2'-deoxyuridine, 5'-O-DMT protected; 5'-O-(4,4'-Dimethoxytrityl)-5-fluoro-O4-(mesityl)-2'-deoxyuridine; N/A
EINECS	Contact for details

Quality Control

Our production of this high-value nucleoside derivative adheres to stringent quality control protocols to ensure batch-to-batch consistency and purity suitable for sensitive oligonucleotide synthesis. Each lot is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry. Comprehensive Certificates of Analysis (COA) detailing identity, purity, and impurity profiles are provided with every shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) in a desiccated environment to prevent degradation due to moisture sensitivity. Allow the sealed container to equilibrate to room temperature before opening to minimize condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.