Description

n-Isobutyryl-3'-O-(4,4'-Dimethoxytrityl)-2'-Deoxyguanosine, 97% is a high-purity, chemically modified nucleoside derivative, specifically a protected deoxyguanosine building block. This compound is critical for ensuring high coupling efficiency and sequence fidelity in automated oligonucleotide synthesis. It is an essential raw material for research and production in the fields of therapeutic oligonucleotides, antisense drugs, and molecular diagnostics.

Application

• Solid-Phase Oligonucleotide Synthesis (SPOS): A key phosphoramidite precursor for the automated synthesis of DNA and RNA strands.
• Antisense Oligonucleotide (ASO) Manufacturing: Used in the production of therapeutic ASOs for targeting specific mRNA sequences.
• siRNA and miRNA Synthesis: Serves as a protected guanosine monomer for constructing short interfering RNA (siRNA) and microRNA (miRNA) molecules.
• PCR Primer and Probe Development: Enables the synthesis of high-purity primers and fluorescent probes for diagnostic assays.
• Research-Grade Oligo Production: Employed in academic and industrial R&D for creating custom oligonucleotides for various studies.
• Next-Generation Sequencing (NGS): Used in the preparation of specialized oligonucleotide libraries and adapters.
• Aptamer Development: A fundamental building block in the synthesis of nucleic acid aptamers for therapeutic and diagnostic applications.

Basic Information

Product Name	n-Isobutyryl-3'-O-(4,4'-Dimethoxytrityl)-2'-Deoxyguanosine, 97%
CAS No.	140712-81-8
Molecular Formula	C41H41N5O8
Molecular Weight	731.80 g/mol
Synonyms	2'-Deoxyguanosine, N2-isobutyryl-3'-O-(4,4'-dimethoxytrityl)-; 3'-O-(4,4'-Dimethoxytrityl)-N2-isobutyryl-2'-deoxyguanosine; N-Isobutyryl-3'-O-DMT-2'-deoxyguanosine; dG(ib)-DMT; N2-Isobutyryl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxyguanosine; Protected Deoxyguanosine Monomer; DMT-dG(ib) Amidite Precursor; Guanosine, 2'-deoxy-, N2-(2-methylpropanoyl)-, 3'-(4,4'-dimethoxytrityl) ether
EINECS	Contact for details

Quality Control

Our n-Isobutyryl-3'-O-(4,4'-Dimethoxytrityl)-2'-Deoxyguanosine is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the stringent requirements for oligonucleotide synthesis. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee traceability and batch-to-batch consistency.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label or COA. This material is hygroscopic (moisture-sensitive); keep the container tightly sealed in a dry environment to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (IR)	Conforms
Assay (HPLC)	≥ 97.0%
Water Content (KF)	≤ 1.0%
Related Substances (HPLC)	Total impurities ≤ 3.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.