Description

N6-Benzoyl-3'-o-(4,4'-Dimethoxytrityl)-2'-Deoxyadenosine is a high-purity, protected nucleoside phosphoramidite precursor, a critical building block in automated oligonucleotide synthesis. Its value lies in enabling the precise and efficient assembly of DNA sequences for research, diagnostics, and therapeutic development. This compound is essential for pharmaceutical R&D teams, diagnostic kit manufacturers, and academic laboratories focused on molecular biology, antisense technology, and next-generation sequencing platforms.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite monomer in solid-phase synthesis for research-scale and GMP-grade DNA/RNA production.
• Antisense & siRNA Therapeutics: Precursor for manufacturing therapeutic oligonucleotides targeting specific genes.
• Diagnostic Probe Development: Used to synthesize labeled probes for PCR, microarrays, and fluorescence in situ hybridization (FISH).
• Next-Generation Sequencing (NGS): Building block for constructing custom primers, adapters, and barcodes used in sequencing workflows.
• Molecular Biology Research: Essential reagent for creating gene fragments, site-directed mutagenesis primers, and synthetic genes.
• CRISPR/Cas9 System Components: Synthesis of guide RNAs (gRNAs) and donor DNA templates for genome editing applications.

Basic Information

Product Name	N6-Benzoyl-3'-o-(4,4'-Dimethoxytrityl)-2'-Deoxyadenosine
CAS No.	140712-79-4
Molecular Formula	C44H39N5O7
Molecular Weight	749.82 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N6-benzoyl-2'-deoxyadenosine; dA(Bz)-DMTr; N6-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxyadenosine; 2'-Deoxyadenosine, N6-benzoyl-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-; Protected 2'-Deoxyadenosine Phosphoramidite Precursor; DMTr-dA(Bz) (common abbreviation); Bz-dA(DMTr)
EINECS	Contact for details

Quality Control

Our N6-Benzoyl-3'-o-(4,4'-Dimethoxytrityl)-2'-Deoxyadenosine is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for sensitive oligonucleotide synthesis. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and moisture content. We support compliance with GMP, ICH Q7 guidelines, and relevant pharmacopeial standards for advanced pharmaceutical intermediates.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions or a dry inert atmosphere to maintain stability and purity. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, DMF, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.