Description

4'-Azidothymidine 5'-Triphosphate (CAS NO 140158-13-0) is a modified nucleoside triphosphate analog, specifically engineered for advanced biochemical research and diagnostic applications. This compound is critical for its role in click chemistry and photoaffinity labeling, enabling precise tracking and manipulation of nucleic acid processes. It is an essential reagent for researchers and developers in the fields of molecular biology, enzymology, and next-generation sequencing technology development.

Application

• Click Chemistry Reagent: Used in Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) reactions for bioconjugation and labeling of nucleic acids.
• Photoaffinity Labeling: Serves as a photoactivatable crosslinker to study protein-nucleic acid interactions and enzyme active sites.
• DNA/RNA Sequencing: Acts as a terminator or modified substrate in novel sequencing-by-synthesis (SBS) platforms.
• Enzyme Mechanism Studies: Utilized as a substrate analog to investigate the kinetics and mechanisms of DNA polymerases and reverse transcriptases.
• Probe Development: Incorporated into oligonucleotide probes for sensitive detection and imaging applications.
• Antiviral Research: Investigated for its potential as a chain terminator in the study of viral replication mechanisms.

Basic Information

Product Name	4'-Azidothymidine 5'-Triphosphate
CAS No.	140158-13-0
Molecular Formula	C10H15N6O13P3
Molecular Weight	520.17 g/mol
Synonyms	AZT-TP; 3'-Azido-3'-deoxythymidine 5'-triphosphate; Azidothymidine triphosphate; 5'-Triphosphoric acid, 3'-azido-3'-deoxythymidine; 3'-Azido-2',3'-dideoxythymidine 5'-triphosphate; 3'-Azido-3'-deoxy-5'-O-[(phosphonooxy)phosphinyl]thymidine; AZT 5'-Triphosphate; 3'-N3-TTP
EINECS	Contact for details

Quality Control

Our 4'-Azidothymidine 5'-Triphosphate is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity for research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and stability. We adhere to ISO 9001 quality management principles, and our analytical methods, including HPLC and NMR, are validated to provide reliable specifications for our customers.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, it is recommended to keep the material desiccated and under an inert atmosphere to maintain stability.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Biological Activity	Functional in enzymatic assays (as reported)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.