Description

2’-Deoxy-2’-Fluoro-3’,5’-Bis-o-Tbdms-Uridine is a key protected nucleoside intermediate, specifically designed for advanced oligonucleotide synthesis. Its primary value lies in the strategic protection of the 3' and 5' hydroxyl groups with TBDMS, which is crucial for the controlled, stepwise assembly of fluorinated antisense oligonucleotides and siRNA. This high-purity building block is essential for pharmaceutical R&D and manufacturing teams focused on developing next-generation therapeutic nucleic acids, including those for antiviral and oncology applications.

Application

• Solid-Phase Oligonucleotide Synthesis (SPOS): A critical phosphoramidite precursor for the automated synthesis of fluorinated oligonucleotides.
• Antisense Oligonucleotide (ASO) Development: Used in the manufacture of ASOs with enhanced nuclease resistance and binding affinity due to the 2'-fluoro modification.
• siRNA and miRNA Therapeutics: Serves as a protected monomer for constructing siRNA strands, improving stability and pharmacokinetic profiles.
• Pharmaceutical Research: A vital building block in medicinal chemistry for exploring novel nucleic acid-based drug candidates.
• Diagnostic Probe Synthesis: Employed in creating labeled probes for genetic testing and molecular diagnostics.
• Process Development & Scale-Up: Supports GMP-compliant manufacturing processes for clinical and commercial oligonucleotide APIs.

Basic Information

Product Name	2’-Deoxy-2’-Fluoro-3’,5’-Bis-o-Tbdms-Uridine
CAS No.	139878-36-7
Molecular Formula	C21H39FN2O6Si2
Molecular Weight	502.72 g/mol
Synonyms	2'-Deoxy-2'-fluoro-3',5'-bis-O-(tert-butyldimethylsilyl)uridine; 2'-Fluoro-3',5'-bis-O-(tert-butyldimethylsilyl)-2'-deoxyuridine; 2'-F-3',5'-bis-O-TBDMS-Uridine; 1-[(2R,3R,4R,5R)-3-Fluoro-4-hydroxy-5-[hydroxy-bis(1,1-dimethylethyl)silyl]oxy-tetrahydrofuran-2-yl]-5-methyl-pyrimidine-2,4-dione 3',5'-bis-O-TBDMS derivative; 2'-Fluoro-2'-deoxy-3',5'-bis-O-(tert-butyldimethylsilyl)uridine; Uridine, 2'-deoxy-2'-fluoro-3',5'-bis-O-[(1,1-dimethylethyl)dimethylsilyl]-; Protected 2'-Fluoro-2'-deoxyuridine; 2'-F-dU(TBDMS)2
EINECS	Contact for details

Quality Control

Our 2’-Deoxy-2’-Fluoro-3’,5’-Bis-o-Tbdms-Uridine is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and Karl Fischer titration for moisture content, to ensure it meets the stringent requirements for pharmaceutical intermediate use. Certificates of Analysis (COA) with full traceability are provided for every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label. This compound is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Related Substances (HPLC)	Total impurities ≤ 2.0% Any single impurity ≤ 1.0%
Residual Solvents (GC)	Meets ICH Q3C guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.