Description

3-n-Boc-5''-o-Dimethoxytrityl-3''-Fluoro-Thymidine is a sophisticated nucleoside derivative, specifically engineered as a key building block in the synthesis of modified oligonucleotides. This compound matters because it provides researchers and manufacturers with a protected, fluorinated thymidine analog, enabling precise control over nucleic acid structure and function. It is primarily needed by pharmaceutical R&D teams and fine chemical suppliers engaged in the development of antisense oligonucleotides, siRNA therapeutics, and diagnostic probes.

Application

• Oligonucleotide Synthesis: A critical phosphoramidite precursor for the solid-phase synthesis of fluorinated oligonucleotides.
• Antisense Drug Development: Used in the research and production of antisense oligonucleotides (ASOs) for therapeutic applications.
• siRNA and miRNA Therapeutics: Serves as a modified building block to enhance the stability and efficacy of RNA-based therapeutics.
• Diagnostic Probe Manufacturing: Incorporated into fluorescent or quencher-labeled probes for molecular diagnostics and hybridization assays.
• Structural Biology Studies: Utilized to investigate the impact of fluorine substitution on DNA/RNA duplex stability and conformation.
• Academic and Industrial Research: A valuable reagent for chemical biology and medicinal chemistry programs exploring nucleic acid modifications.

Basic Information

Product Name	3-n-Boc-5''-o-Dimethoxytrityl-3''-Fluoro-Thymidine
CAS No.	138685-99-1
Molecular Formula	C36H39FN2O8
Molecular Weight	646.71 g/mol
Synonyms	3'-Fluoro-5'-O-(4,4'-dimethoxytrityl)-3-N-[(tert-butoxy)carbonyl]thymidine; 3'-Fluoro-3-N-Boc-5'-O-DMT-thymidine; 3'-F-5'-O-DMT-3-N-Boc-dT; 1-[(2R,4S,5R)-4-Fluoro-5-[(4,4'-dimethoxytrityl)oxymethyl]tetrahydrofuran-2-yl]-5-methyl-3-[(2-methylpropan-2-yl)oxycarbonyl]pyrimidine-2,4-dione; 3-N-Boc-5'-O-DMT-3'-fluoro-2'-deoxythymidine; 3'-Fluoro-2'-deoxythymidine, 3-N-Boc-5'-O-DMT protected; 3'-F-dT(Boc,DMT)
EINECS	Contact for details

Quality Control

Our production of 3-n-Boc-5''-o-Dimethoxytrityl-3''-Fluoro-Thymidine adheres to stringent quality protocols suitable for research and pharmaceutical intermediate applications. Each batch undergoes rigorous analytical testing, including HPLC for purity and NMR for structural confirmation, to ensure consistency and reliability. Certificates of Analysis (COA) detailing identity, purity, and impurity profiles are available upon request to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label/Certificate of Analysis. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.