Description

(5-Azido-2-Nitrobenzoyl)Spermine is a specialized bifunctional chemical reagent that combines a photoreactive azido group with a nitroaromatic moiety and a polyamine spermine backbone. This unique structure makes it a valuable tool for photoaffinity labeling and cross-linking studies in biochemical research. It is primarily used by researchers in pharmaceutical development, proteomics, and molecular biology who require precise tools for investigating protein-ligand interactions and biomolecular structures. The compound offers a controlled method for covalent attachment under UV light, enabling the study of complex biological systems.

Application

• Photoaffinity Labeling Probe: Used to covalently tag and identify binding sites of proteins, receptors, and enzymes upon UV irradiation.
• Bioconjugation and Cross-linking Agent: Facilitates the study of protein-protein interactions and the construction of biomolecular assemblies.
• Proteomics Research: Employed in mass spectrometry-based workflows for mapping protein surfaces and interaction networks.
• Drug Discovery Target Identification: Aids in the elucidation of molecular targets for novel therapeutic compounds.
• Biomaterial Functionalization: Used to modify surfaces with bioactive molecules for biosensor and diagnostic device development.
• Nucleic Acid Research: Potential application in cross-linking studies involving DNA and RNA binding proteins.

Basic Information

Product Name	(5-Azido-2-Nitrobenzoyl)Spermine
CAS No.	138109-67-8
Molecular Formula	C21H28N8O3
Molecular Weight	440.50 g/mol
Synonyms	N1,N12-Bis(5-azido-2-nitrobenzoyl)spermine; 5-Azido-2-nitrobenzoic Acid Spermine Bisamide; Spermine, N,N'-bis(5-azido-2-nitrobenzoyl)-; Azidonitrobenzoylspermine; Photoaffinity Spermine Derivative; ANB-Spermine; Bifunctional Spermine Crosslinker
EINECS	Contact for details

Quality Control

Our (5-Azido-2-Nitrobenzoyl)Spermine is produced under strict quality management protocols to ensure batch-to-batch consistency and high purity for research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and stability data. We adhere to relevant standards for fine chemical and biochemical reagent production to support your critical research and development projects.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere for long-term stability. This compound is light-sensitive (store away from light) and should be handled under subdued lighting conditions. Allow the container to reach room temperature in a dark environment before opening to prevent condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.