Description

5'-O-(Dimethoxytrityl)-8-Hydroxy-N2-Isobutyryl-2'-Deoxyguanosine is a key protected nucleoside phosphoramidite precursor, essential for the automated solid-phase synthesis of oligonucleotides. This compound matters because it provides the critical guanine base with orthogonal protecting groups, ensuring high coupling efficiency and purity in the final DNA or RNA sequence. It is primarily needed by manufacturers and research institutions in the pharmaceutical, biotechnology, and diagnostic sectors for developing therapeutics, probes, and primers.

Application

• Oligonucleotide Synthesis: As a phosphoramidite building block for the automated synthesis of DNA and RNA strands.
• Antisense Drug Development: For the production of antisense oligonucleotides (ASOs) used in therapeutic applications.
• PCR Primer and Probe Manufacture: Enables the creation of high-purity primers and fluorescent probes for molecular diagnostics.
• Research & Development: Used in academic and industrial labs for studying gene function, gene silencing (RNAi), and nucleic acid chemistry.
• Next-Generation Sequencing (NGS): Serves as a reagent in the preparation of sequencing libraries and custom oligo pools.
• Therapeutic Aptamer Production: A critical raw material in the synthesis of nucleic acid aptamers for targeted therapies.

Basic Information

Product Name	5'-O-(Dimethoxytrityl)-8-Hydroxy-N2-Isobutyryl-2'-Deoxyguanosine
CAS No.	136859-77-3
Molecular Formula	C41H43N5O8
Molecular Weight	733.82 g/mol
Synonyms	8-Hydroxy-N2-Isobutyryl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxyguanosine; 5'-O-DMT-8-OH-N2-ibu-dG; dG(Ibu,8-OH) Phosphoramidite precursor; 2'-Deoxyguanosine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-8-hydroxy-N-(2-methyl-1-oxopropyl)-; Protected 2'-Deoxyguanosine Derivative; N2-Isobutyryl-8-oxo-2'-deoxyguanosine DMT ether; Guanosine, 2'-deoxy-, 5'-(4,4'-dimethoxytrityl) ether, 8-hydroxy, N2-isobutyrate
EINECS	Contact for details

Quality Control

Our 5'-O-(Dimethoxytrityl)-8-Hydroxy-N2-Isobutyryl-2'-Deoxyguanosine is manufactured under strict quality systems to ensure it meets the high-purity standards required for sensitive oligonucleotide synthesis. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and moisture content. We adhere to cGMP guidelines where applicable to support its use in pharmaceutical development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) in a dry, moisture-free environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, DMF, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.