Description

Cytidine-2-14C 5'-Triphosphate Ammonium Salt is a radiolabeled nucleotide essential for tracing and quantifying RNA synthesis and metabolism in biochemical research. This compound is critical for high-sensitivity studies requiring precise detection of nucleic acid incorporation and enzymatic activity. It is primarily utilized by research scientists in pharmaceutical development, molecular biology, and biochemistry for applications such as in vitro transcription assays and metabolic pathway analysis.

Application

• Biochemical Research & Enzymology: Used as a radiolabeled substrate for studying RNA polymerase, reverse transcriptase, and other nucleotide-dependent enzyme kinetics and mechanisms.
• Molecular Biology Assays: Essential for sensitive in vitro transcription assays, primer extension analyses, and the synthesis of radiolabeled RNA probes.
• Metabolic Pathway Tracing: Enables the tracking of cytidine nucleotide incorporation into RNA, facilitating studies on RNA synthesis, turnover, and cellular metabolism.
• Pharmaceutical R&D: Employed in drug discovery to screen for and evaluate inhibitors of viral replication (e.g., HIV, HCV) that target RNA-dependent processes.
• Autoradiography & Imaging: Serves as a tracer for autoradiographic techniques to visualize and localize RNA synthesis in tissues, cells, or gels.

Basic Information

Product Name	Cytidine-2-14C 5'-Triphosphate Ammonium Salt
CAS No.	134940-99-1
Molecular Formula	C9H17N3O14P3 • xNH3
Molecular Weight	Approx. 503.1 (unlabeled, free acid) + labeled mass. Contact for details.
Synonyms	CTP-2-14C Ammonium Salt; [2-14C]CTP Ammonium Salt; Cytidine 5'-(tetrahydrogen triphosphate), 2-14C-labeled, ammonium salt; 2-14C-CTP; Radiolabeled CTP; [2-14C]Cytidine-5'-triphosphate ammonium salt; 5'-CTP-2-14C
EINECS	Contact for details.

Quality Control

Every batch of Cytidine-2-14C 5'-Triphosphate Ammonium Salt is manufactured and tested under stringent quality systems to ensure the highest standards of radiochemical purity and specific activity. Our quality commitment includes comprehensive analytical testing via HPLC and spectroscopic methods. A detailed Certificate of Analysis (COA), specifying radiochemical purity, specific activity, and chemical purity, is provided with each shipment to ensure traceability and compliance with your research protocols.

Storage

Preserve in a tightly closed container, protected from light. Store frozen (≤ -20°C) or as recommended on the product label or COA. Due to its hygroscopic (moisture-sensitive) nature, the product should be handled under anhydrous conditions and allowed to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or solution
Identification (HPLC, MS)	Conforms to reference standard
Radiochemical Purity (HPLC)	≥ 95%
Specific Activity	50-60 mCi/mmol (Contact for exact value)
Chemical Purity (HPLC)	≥ 90%
Solvent (if supplied as solution)	Contact for details
pH (if in aqueous solution)	6.5 - 7.5

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.