Description

Adenosine-8-14C 5'-Diphosphate Ammonium is a radiolabeled nucleotide analog essential for tracing and quantifying biochemical pathways involving ATP and energy metabolism. This compound is critical for researchers requiring high-specific-activity tracers to study enzyme kinetics, nucleotide transport, and receptor-ligand interactions with precision. It is primarily utilized by pharmaceutical R&D teams, academic biochemistry laboratories, and diagnostic developers focused on metabolic and cardiovascular research.

Application

• Enzyme Kinetics & Mechanism Studies: Used as a radiolabeled substrate for investigating the activity and inhibition of kinases, ATPases, and other nucleotide-dependent enzymes.
• Metabolic Pathway Tracing: Employed in tracer studies to elucidate ATP utilization, energy charge, and nucleotide recycling in cellular systems.
• Receptor Binding Assays: Serves as a high-specific-activity ligand for characterizing purinergic receptors (P2Y, P2X families) in pharmacological research.
• Diagnostic Probe Development: Acts as a key intermediate in the synthesis of radiolabeled probes for molecular imaging and diagnostic applications.
• Biochemical Standard: Provides a calibrated reference standard for the quantification of ADP and related nucleotides via LC-MS or scintillation counting.
• Cardiovascular & Neurological Research: Applied in studies exploring the role of extracellular nucleotides in platelet aggregation, vasodilation, and neurotransmission.

Basic Information

Product Name	Adenosine-8-14C 5'-Diphosphate Ammonium
CAS No.	134907-90-7
Molecular Formula	C10H14N5O10P2 • xNH3
Molecular Weight	~427.2 (Free acid), plus ammonium. Contact for exact weight of salt form.
Synonyms	8-14C-ADP Ammonium Salt; Adenosine-5'-diphosphate-8-14C ammonium; [8-14C]ADP ammonium salt; 8-Carbon-14-labeled ADP ammonium; Adenosine 5'-(trihydrogen diphosphate), 8-14C-labeled, ammonium salt; 8-14C-Adenosine diphosphate ammonium; ADP-8-14C ammonium salt; 5'-ADP-8-14C ammonium
EINECS	Contact for details.

Quality Control

Every batch of Adenosine-8-14C 5'-Diphosphate Ammonium is manufactured and tested under strict quality systems to ensure identity, purity, and specific activity. Our quality commitment includes comprehensive analytical testing via HPLC (UV and Radiometric detection), TLC, and NMR to confirm chemical and radiochemical purity. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting exact specifications, specific activity (mCi/mmol), and solvent composition.

Storage

Preserve in a tightly closed container, protected from light. Store frozen (-20°C or below) in the original packaging. This product is hygroscopic (moisture-sensitive). For long-term stability, store under inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or frozen solution
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Chemical Purity (HPLC-UV)	≥ 95%
Radiochemical Purity (HPLC)	≥ 97%
Specific Activity	50-60 mCi/mmol (Contact for exact value)
Solvent	Contact for details (typically aqueous buffer or ethanol:water)
Concentration	1.0 mCi/mL (Nominal, contact for exact)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.