Description

2-((4-Azido-2-Nitrophenyl)Amino)Propyl Triphosphate is a specialized nucleotide analog featuring a photoreactive azido group and a triphosphate moiety. This compound is critical for applications requiring photoaffinity labeling and cross-linking in biochemical research. It is primarily utilized by researchers and manufacturers in the fields of molecular biology, enzymology, and proteomics. The product, identified by CAS No. 133338-54-2, is supplied with a focus on high purity and stability for sensitive experimental protocols.

Application

• Photoaffinity Labeling: Acts as a key reagent for covalently tagging and identifying nucleotide-binding sites on proteins and enzymes.
• Enzyme Mechanism Studies: Used to probe the active sites and catalytic mechanisms of ATP-dependent enzymes and kinases.
• Proteomics Research: Enables the identification and characterization of novel protein targets that interact with nucleotide triphosphates.
• Cross-linking Agent: Facilitates the study of protein-nucleic acid and protein-protein interactions through UV-induced cross-linking.
• Bioconjugation: Serves as a building block for synthesizing advanced probes and conjugates for diagnostic assay development.
• Drug Discovery: Supports target validation and screening efforts for therapeutic programs focused on nucleotide-binding proteins.

Basic Information

Item	Details
Product Name	2-((4-Azido-2-Nitrophenyl)Amino)Propyl Triphosphate
CAS No.	133338-54-2
Molecular Formula	C9H13N6O11P3
Molecular Weight	474.16 g/mol
Synonyms	ANP-Triphosphate; 2-[(4-Azido-2-nitrophenyl)amino]propyl triphosphate; 4-Azido-2-nitrophenylaminopropyl triphosphate; N-(4-Azido-2-nitrophenyl)-2-aminopropyl triphosphate; Photoreactive ATP analog; ANPP-Triphosphate; Photoaffinity labeling nucleotide; Contact for details on additional trade names.
EINECS	Contact for details

Quality Control

Our 2-((4-Azido-2-Nitrophenyl)Amino)Propyl Triphosphate is manufactured under strict quality protocols to ensure batch-to-batch consistency and high chemical purity suitable for research applications. Each lot is analyzed to confirm identity, purity, and the absence of critical impurities. A comprehensive Certificate of Analysis (COA) detailing specifications such as assay (HPLC), absorbance profile, and moisture content is provided with every shipment to support your quality assurance requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is both light-sensitive and hygroscopic. For long-term stability, it is recommended to store under an inert atmosphere and avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Yellow to orange solid or solution
Identification (UV-Vis)	Conforms
Purity (HPLC)	≥ 95%
Absorbance (λ max)	~470 nm (characteristic)
Water Content (KF)	≤ 2.0%
Solubility	Soluble in aqueous buffers, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.