Description

2'-Deoxy-2'-Fluoro-5'-o-(4,4'-Dimethoxytrityl)-5-Methyluridine is a key nucleoside phosphoramidite building block, specifically a 5-methyluridine derivative protected with a dimethoxytrityl (DMT) group at the 5'-hydroxyl position. This compound is essential for the solid-phase synthesis of oligonucleotides, particularly for introducing modified bases that enhance stability and binding affinity. It is critically required by pharmaceutical R&D teams and fine chemical manufacturers engaged in the development of therapeutic nucleic acids, including antisense oligonucleotides and siRNA.

Application

• Oligonucleotide Synthesis: Primary building block (phosphoramidite) for the automated solid-phase synthesis of modified DNA/RNA strands.
• Antisense Drug Development: Used to incorporate 2'-fluoro-5-methyluridine modifications, which increase nuclease resistance and improve target binding for antisense therapeutic candidates.
• siRNA and miRNA Research: Critical for manufacturing chemically modified siRNA duplexes to enhance pharmacokinetic properties and efficacy in gene silencing applications.
• Diagnostic Probe Manufacture: Employed in the production of labeled probes and primers with improved thermal stability for PCR and molecular diagnostics.
• Pharmaceutical Intermediates: Serves as a high-purity advanced intermediate in the multi-step synthesis of novel nucleoside-based antiviral or anticancer agents.
• Biochemical Research: Utilized in academic and industrial labs to study structure-activity relationships (SAR) of nucleic acids and their interactions with proteins.

Basic Information

Product Name	2'-Deoxy-2'-Fluoro-5'-o-(4,4'-Dimethoxytrityl)-5-Methyluridine
CAS No.	133324-02-4
Molecular Formula	C31H31FN2O7
Molecular Weight	562.59 g/mol
Synonyms	5-Methyl-2'-deoxy-2'-fluorouridine 5'-O-(4,4'-dimethoxytrityl) ether; 5'-O-DMT-2'-F-5-methyluridine; 2'-Fluoro-5-methyluridine 5'-O-DMT ether; 1-[(2R,3R,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-methyl-1,2,3,4-tetrahydropyrimidine-2,4-dione 5'-(4,4'-dimethoxytrityl) ether; DMT-2'F-5MeU; 2'-F-5-Me-U DMT; NSC 758452; 5-Methyl-2'-fluoro-2'-deoxyuridine 5'-O-DMT
EINECS	Contact for details

Quality Control

Our 2'-Deoxy-2'-Fluoro-5'-o-(4,4'-Dimethoxytrityl)-5-Methyluridine is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and LC-MS for identity verification, to ensure it meets the exacting standards required for oligonucleotide synthesis. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee traceability and batch-to-batch consistency for your critical R&D and cGMP manufacturing processes.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, freezer. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with reference structure
Purity (HPLC)	≥ 98.0% (Area Percent)
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.