Description

5-(((2,2,5,5-Tetramethyl-1-Oxy-3-Pyrrolidinyl)Amino)Methyl)-2'-Deoxyuridine is a specialized nitroxide spin-labeled nucleoside derivative, serving as a critical molecular probe for advanced biophysical research. Its core value lies in enabling the study of nucleic acid dynamics, structure, and interactions through techniques like Electron Paramagnetic Resonance (EPR) spectroscopy. This compound is essential for researchers and developers in structural biology, biophysics, and medicinal chemistry who require precise tools for investigating DNA and RNA behavior at the molecular level.

Application

• EPR/ESR Spectroscopy Probe: Acts as a site-directed spin label for studying the dynamics, conformation, and flexibility of DNA and oligonucleotides.
• Nucleic Acid Research: Used to investigate DNA-protein interactions, enzyme mechanisms (e.g., polymerases, nucleases), and nucleic acid folding.
• Structural Biology: Aids in determining distance constraints within large biomolecular complexes via pulsed EPR techniques like DEER (PELDOR).
• Drug Discovery: Employed in screening and mechanistic studies of compounds targeting nucleic acids, such as anticancer or antiviral agents.
• Biophysical Labeling: Facilitates the real-time monitoring of conformational changes in DNA upon binding with ligands, metals, or other biomolecules.
• Diagnostic Assay Development: Serves as a component in advanced diagnostic platforms and biosensors that utilize spin-label detection methods.

Basic Information

Product Name	5-(((2,2,5,5-Tetramethyl-1-Oxy-3-Pyrrolidinyl)Amino)Methyl)-2'-Deoxyuridine
CAS No.	132723-74-1
Molecular Formula	C16H26N4O6
Molecular Weight	370.40 g/mol
Synonyms	5-(Aminomethyl)-2'-deoxyuridine TEMPO derivative; 5-((2,2,5,5-Tetramethyl-1-oxyl-3-pyrrolidinyl)aminomethyl)-2'-deoxyuridine; 2'-Deoxy-5-(((2,2,5,5-tetramethyl-1-oxy-3-pyrrolidinyl)amino)methyl)uridine; Spin-labeled 2'-deoxyuridine; TEMPO-dU; dU-TEMPO; Nitroxide-labeled deoxyuridine; 5-(TEMPO-aminomethyl)-2'-deoxyuridine
EINECS	Contact for details

Quality Control

Our 5-(((2,2,5,5-Tetramethyl-1-Oxy-3-Pyrrolidinyl)Amino)Methyl)-2'-Deoxyuridine is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency, critical for sensitive research applications. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant quality guidelines for research chemicals, with specifications tailored to meet the stringent requirements of EPR spectroscopy and biophysical studies.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) for long-term stability. This compound is easily oxidized and light-sensitive; prolonged exposure to air, light, or elevated temperatures should be avoided. Allow the sealed container to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Off-white to light yellow solid
Identification (HPLC/MS)	Conforms
Identification (IR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines
EPR Activity	Confirmed

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.