Description

2'-o-Propargyluridine is a modified nucleoside featuring a propargyl group at the 2'-oxygen position of the ribose sugar. This specific chemical modification is crucial for its role as a key building block in oligonucleotide synthesis, particularly for click chemistry applications. It is primarily utilized by researchers and manufacturers in the fields of pharmaceutical development, diagnostic probe design, and life science research to create advanced nucleic acid-based tools and therapeutics.

Application

• Oligonucleotide Synthesis: A critical phosphoramidite precursor for the solid-phase synthesis of modified RNA and DNA strands.
• Click Chemistry Conjugation: The alkyne (propargyl) group enables efficient, site-specific bioconjugation via copper-catalyzed azide-alkyne cycloaddition (CuAAC) for labeling.
• Antisense & siRNA Therapeutics: Used to introduce modifications that can enhance stability, specificity, and delivery of therapeutic oligonucleotides.
• Diagnostic Probe Development: Incorporation into probes for fluorescence in situ hybridization (FISH) or other detection platforms to improve signal and reduce background.
• Chemical Biology Research: A versatile tool for studying RNA structure, function, and metabolism through bioorthogonal labeling strategies.
• Aptamer Engineering: Modification of nucleic acid aptamers to improve their binding affinity and nuclease resistance for therapeutic and sensing applications.

Basic Information

Item	Detail
Product Name	2'-o-Propargyluridine
CAS No.	129778-58-1
Molecular Formula	C12H12N2O6
Molecular Weight	280.23 g/mol
Synonyms	2'-O-Propargyluridine; 1-[(2R,3R,4R,5R)-3,4-Dihydroxy-5-(hydroxymethyl)-2-(prop-2-yn-1-yloxy)oxolan-2-yl]pyrimidine-2,4-dione; 2'-O-(2-Propynyl)uridine; Uridine, 2'-O-(2-propynyl)-; Propargyluridine; 2'-O-Propargyl-Uridine; 2'-O-Prg-U
EINECS	Contact for details

Quality Control

Our 2'-o-Propargyluridine is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency, suitable for sensitive research and development applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We support compliance with relevant guidelines for advanced pharmaceutical intermediates.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This compound is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.