Description

2''-(Adenosine-5'-Thiophosphoryl)Tobramycin is a chemically modified derivative of the aminoglycoside antibiotic tobramycin, where a thiophosphoryl adenosine moiety is covalently linked. This modification is strategically designed to create a novel molecular probe or a potential prodrug, offering unique biochemical properties distinct from the parent compound. It is primarily utilized by researchers and developers in pharmaceutical R&D and academic biochemistry for studying antibiotic mechanisms, enzyme interactions, and targeted drug delivery systems.

Application

• Biochemical Research: As a specialized probe for studying ribosome interactions, RNA binding, and the mechanisms of action of aminoglycoside antibiotics.
• Enzymology Studies: Investigation of kinase and phosphoryl transferase activities due to its thiophosphoryl group, which can act as a substrate or inhibitor.
• Prodrug Development: Serves as a precursor molecule in the design of novel antibiotic prodrugs with potentially altered pharmacokinetics or reduced toxicity.
• Molecular Biology Tools: Used in the development of affinity tags or cross-linking agents for protein and nucleic acid isolation and analysis.
• Antimicrobial Resistance Research: A key compound for exploring new pathways to overcome bacterial resistance mechanisms to conventional aminoglycosides.

Basic Information

Product Name	2''-(Adenosine-5'-Thiophosphoryl)Tobramycin
CAS No.	129545-98-8
Molecular Formula	C27H42N11O12PS
Molecular Weight	775.74 g/mol
Synonyms	Tobramycin 2''-adenosine-5'-thiophosphate; 2''-O-(5'-Thiophosphoadenosine)tobramycin; Adenosine 5'-(P-thio)-2''-tobramycin ester; 5'-Thiophosphoryladenosine-2''-tobramycin; Tobramycin adenosine thiophosphate conjugate; APT-Tobramycin; Thiophosphorylated Tobramycin-Adenosine Conjugate
EINECS	Contact for details

Quality Control

Our 2''-(Adenosine-5'-Thiophosphoryl)Tobramycin is produced and handled under strict quality management protocols. Each batch undergoes comprehensive analytical characterization to ensure identity, purity, and consistency. Certificates of Analysis (COA) detailing results from HPLC, NMR, and MS are provided to support research and development applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. Due to its hygroscopic (moisture-sensitive) nature, containers should be allowed to equilibrate to room temperature before opening to minimize condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.