Description

3'-Tbdms-Bz-Rc Phosphoramidite is a high-purity, specialty phosphoramidite monomer used in the automated solid-phase synthesis of oligonucleotides. This compound is critical for introducing specific, protected nucleoside building blocks into DNA or RNA sequences with high coupling efficiency and fidelity. It is an essential raw material for research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in the development of therapeutics, probes, and primers.

Application

• Oligonucleotide Synthesis: As a key building block (monomer) in automated solid-phase synthesizers for the production of custom DNA and RNA strands.
• Therapeutic Development: For the synthesis of antisense oligonucleotides (ASOs), siRNA, and aptamers used in drug discovery and development.
• Diagnostic Probes: Manufacturing of labeled and unlabeled probes for PCR, qPCR, FISH, and other molecular diagnostic applications.
• Research Reagents: Synthesis of primers, linkers, and gene fragments for academic and industrial life science research.
• Synthesis of Modified Oligos: Enabling the incorporation of base-modified or backbone-modified nucleotides for advanced biochemical studies.

Basic Information

Product Name	3'-Tbdms-Bz-Rc Phosphoramidite
CAS No.	129470-47-9
Molecular Formula	C41H51N4O7PSi
Molecular Weight	771.01 g/mol
Synonyms	5'-O-DMT-2'-O-TBDMS-N4-Bz-rC Phosphoramidite; 5'-O-(4,4'-Dimethoxytrityl)-2'-O-(tert-butyldimethylsilyl)-N4-benzoylcytidine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 2'-O-TBDMS-N-Bz-CE Phosphoramidite (rC); N4-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-O-[tert-butyl(dimethyl)silyl]cytidine 3'-(2-cyanoethyl N,N-diisopropylphosphoramidite); TBDMS-rC(Bz) Phosphoramidite; RNA Cytidine Phosphoramidite (Bz, 2'-O-TBDMS); 2'-O-TBDMS-N-Bz-C Phosphoramidite
EINECS	Contact for details

Quality Control

Our 3'-Tbdms-Bz-Rc Phosphoramidite is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for critical synthesis workflows. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) with detailed chromatographic data are provided for every shipment, supporting compliance with internal quality standards for pharmaceutical and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This product is hygroscopic (moisture-sensitive) and easily oxidized; prolonged exposure to air, light, or ambient humidity will lead to degradation and reduced coupling efficiency.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Clear, colorless solution in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.