Description

5'-O-(4,4'-Dimethoxy)- 3'-O-(T-Butyldimethylsilyl) Uridine, 2'-[(2-Cyanoethyl)-(N,N-Diisopropyl)]-Phosphoramidite is a highly specialized, protected nucleoside phosphoramidite monomer, a critical building block in automated solid-phase oligonucleotide synthesis. This compound is essential for the precise and efficient assembly of RNA and modified oligonucleotide sequences. It is primarily utilized by research institutions, pharmaceutical developers, and biotechnology companies engaged in therapeutic oligonucleotide discovery, antisense technology, and diagnostic probe development.

Application

• Therapeutic Oligonucleotide Synthesis: A key raw material for synthesizing siRNA, antisense oligonucleotides (ASOs), and aptamers for pharmaceutical applications.
• Research-Grade RNA Synthesis: Used in laboratories for the custom synthesis of RNA strands for molecular biology, virology, and structural biology studies.
• Diagnostic Probe Manufacturing: Essential for producing labeled and modified nucleic acid probes used in PCR, FISH, and microarray technologies.
• Synthesis of Modified Nucleotides: Enables the site-specific incorporation of modifications to study RNA function or enhance oligonucleotide stability and efficacy.
• Process Development & Scale-Up: Serves as a high-purity starting material for developing and scaling GMP-compliant manufacturing processes for oligonucleotide APIs.

Basic Information

Product Name	5'-O-(4,4'-Dimethoxy)- 3'-O-(T-Butyldimethylsilyl) Uridine, 2'-[(2-Cyanoethyl)-(N,N-Diisopropyl)]-Phosphoramidite
CAS No.	129451-77-0
Molecular Formula	C31H51N4O8PSi
Molecular Weight	666.81 g/mol
Synonyms	5'-O-DMT-2'-O-TBDMS-Uridine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite; 2'-O-TBDMS-5'-O-DMT-Uridine 3'-(2-cyanoethyl N,N-diisopropyl)phosphoramidite; DMT-TBDMS-U Phosphoramidite; Uridine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2'-O-[(1,1-dimethylethyl)dimethylsilyl]-3'-O-[2-[[bis(1-methylethyl)amino](2-cyanoethoxy)phosphinyl]ethyl]-; 2'-O-(tert-Butyldimethylsilyl)-5'-O-(4,4'-dimethoxytrityl)uridine 3'-(2-cyanoethyl N,N-diisopropylphosphoramidite)
EINECS	Contact for details

Quality Control

Our 5'-O-(4,4'-Dimethoxy)- 3'-O-(T-Butyldimethylsilyl) Uridine, 2'-[(2-Cyanoethyl)-(N,N-Diisopropyl)]-Phosphoramidite is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency, critical for successful oligonucleotide synthesis. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity (typically ≥98% by HPLC), identity confirmation, and moisture content. Our quality commitment aligns with the standards required for research and pre-clinical development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This compound is hygroscopic (moisture-sensitive) and easily oxidized; prolonged exposure to air, light, or ambient humidity will compromise its reactivity and shelf life.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.