Description

2',3'-Dideoxy-2'-Fluoroadenosine Triphosphate is a modified nucleoside triphosphate analog designed for advanced biochemical and molecular biology research. This compound matters for its role as a chain-terminating substrate in enzymatic synthesis, particularly for studying nucleic acid polymerases and viral replication mechanisms. It is primarily needed by research institutions, pharmaceutical R&D departments, and diagnostic kit manufacturers focused on virology, enzymology, and nucleotide metabolism.

Application

• Enzymatic Synthesis & Polymerase Studies: Used as a substrate analog to investigate the kinetics and fidelity of DNA/RNA polymerases and reverse transcriptases.
• Antiviral Research: Serves as a key biochemical tool in the study of nucleoside analog inhibitors and viral replication pathways.
• Next-Generation Sequencing (NGS): Potential application in specialized sequencing chemistries requiring modified nucleotides.
• Biochemical Assay Development: Employed in the design of high-throughput screening assays for drug discovery targeting nucleic acid enzymes.
• Metabolic Pathway Analysis: Used to trace and study nucleotide incorporation and metabolism in cellular systems.
• Diagnostic Probe Synthesis: Can be utilized in the enzymatic synthesis of specialized probes for molecular diagnostics.

Basic Information

Product Name	2',3'-Dideoxy-2'-Fluoroadenosine Triphosphate
CAS No.	128531-73-7
Molecular Formula	C10H14FN5O12P3
Molecular Weight	529.15 g/mol
Synonyms	2',3'-Dideoxy-2'-fluoro-ATP; 2'-F-ddATP; 2'-Fluoro-2',3'-dideoxyadenosine 5'-triphosphate; 2'-F-2',3'-ddATP; Fluorodideoxy ATP; Adenosine, 2',3'-dideoxy-2'-fluoro-, 5'-(tetrahydrogen triphosphate); 2'-F-ddATP triethylammonium salt (common salt form)
EINECS	Contact for details

Quality Control

Our 2',3'-Dideoxy-2'-Fluoroadenosine Triphosphate is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (NMR, MS), to ensure it meets the high standards required for sensitive research applications. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, it is recommended to keep the material desiccated. Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay (UV)	90 - 110% of stated concentration
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, TE buffer (pH 7.5)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.