Description

3'-Deoxythymidine-5'-o-Triphosphate/2',3'-Dideoxythymidine-5'-o-Triphosphate Sodium Salt is a high-purity nucleotide analog critical for advanced molecular biology and diagnostic applications. This compound serves as a specialized substrate or chain terminator in enzymatic synthesis, enabling precise control in nucleic acid research. It is essential for researchers and manufacturers in the fields of next-generation sequencing (NGS), PCR-based diagnostics, and oligonucleotide synthesis, where reliable quality and consistent performance are paramount.

Application

• DNA Sequencing: Used as a chain-terminating nucleotide (ddNTP) in Sanger sequencing and related methodologies.
• Polymerase Chain Reaction (PCR): Employed in specialized PCR applications requiring controlled termination or labeling.
• Oligonucleotide Synthesis: Acts as a building block in the enzymatic synthesis of modified DNA strands.
• Molecular Diagnostics: A key component in assay kits for pathogen detection, genetic testing, and mutation analysis.
• Biochemical Research: Utilized in studies of DNA polymerase kinetics, fidelity, and enzyme mechanism.
• Antiviral Drug Research: Investigated as a potential nucleotide analog for prodrug development targeting viral polymerases.

Basic Information

Product Name	3'-Deoxythymidine-5'-o-Triphosphate/2',3'-Dideoxythymidine-5'-o-Triphosphate Sodium Salt
CAS No.	128524-26-5
Molecular Formula	C10H14N2Na3O13P3 (or similar, based on sodium salt form)
Molecular Weight	Contact for details
Synonyms	2',3'-Dideoxythymidine-5'-triphosphate sodium salt; ddTTP Na salt; 3'-Deoxythymidine triphosphate sodium; Thymidine 2',3'-dideoxy-5'-triphosphate trisodium salt; ddTTP trisodium; Chain terminator nucleotide; Dideoxynucleotide triphosphate; CAS 128524-26-5
EINECS	Contact for details

Quality Control

Our nucleotide analogs are manufactured under strict quality control protocols suitable for research and diagnostic use. Each batch is tested to ensure high purity and consistency, with Certificates of Analysis (COA) provided. Specifications are designed to meet the stringent requirements of molecular biology applications, with purity typically assessed by HPLC. We support compliance with relevant quality management systems.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, desiccate. Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95% (Typical grades: 95%, 98%, 99%)
Water Content (KF)	≤ 5.0%
pH (in solution)	6.5 - 7.5
Endotoxin	< 10 EU/mg (for molecular biology grade)
Biological Activity	Suitable for enzymatic incorporation

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.