Description

2'-o-Methyl-N2-Dimethylformamide-5'-o-Dmt-Guanosine-3'-Ce-Phosphoramidite is a high-purity, chemically modified phosphoramidite monomer essential for the solid-phase synthesis of oligonucleotides. This compound is specifically engineered for the controlled incorporation of 2'-O-methylguanosine, a key modification that enhances the stability, nuclease resistance, and binding affinity of therapeutic nucleic acids. It is a critical raw material for research institutions and pharmaceutical companies developing advanced antisense oligonucleotides, siRNA, aptamers, and other next-generation genetic medicines.

Application

• Synthesis of 2'-O-Methyl Modified Oligonucleotides: Primary building block for introducing 2'-O-methylguanosine residues into synthetic RNA and DNA strands.
• Therapeutic Nucleic Acid Development: Critical for manufacturing antisense oligonucleotides (ASOs) and small interfering RNA (siRNA) therapeutics with improved pharmacokinetic profiles.
• Aptamer and Diagnostic Probe Production: Used to create high-affinity binding agents and molecular probes with enhanced serum stability for diagnostic and research applications.
• Basic Life Science Research: Enables academic and industrial research into RNA structure, function, and RNA-based mechanisms.
• Synthesis of Splice-Switching Oligonucleotides (SSOs): A key component in the production of SSOs used to modulate gene expression for therapeutic purposes.
• Good Manufacturing Practice (GMP) Production: Suitable for use in GMP-compliant processes for the clinical and commercial-scale manufacturing of oligonucleotide active pharmaceutical ingredients (APIs).

Basic Information

Product Name	2'-o-Methyl-N2-Dimethylformamide-5'-o-Dmt-Guanosine-3'-Ce-Phosphoramidite
CAS No.	128219-77-2
Molecular Formula	C46H53N8O9P
Molecular Weight	893.95 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-O-methyl-N2-(dimethylaminomethylene)guanosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 2'-O-Me-G(Dmf)-CE Phosphoramidite; DMT-2'-O-Methylguanosine(dmf)-CE Phosphoramidite; 2'-O-Methyl G(dmf) Phosphoramidite; Guanosine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2'-O-methyl-N-[(dimethylamino)methylene]-3'-[2-cyanoethoxy(diisopropylamino)phosphino]-; 2'-O-Methyl-N2-DMF-Guanosine Phosphoramidite; 2'-OMe-G(dmf) Phosphoramidite
EINECS	Contact for details

Quality Control

Every batch of our 2'-o-Methyl-N2-Dimethylformamide-5'-o-Dmt-Guanosine-3'-Ce-Phosphoramidite is manufactured and tested under strict quality management systems. We provide comprehensive analytical data to ensure the product meets the stringent purity and consistency requirements for oligonucleotide synthesis. A detailed Certificate of Analysis (COA) is supplied with each shipment, reporting key parameters such as purity (HPLC), identity (NMR, MS), moisture content (KF), and phosphoramidite assay (³¹P NMR). Our quality systems support development from research to GMP-grade material.

Storage

Preserve in a tightly closed container, protected from light. This product is hygroscopic (moisture-sensitive) and should be stored in a freezer at -20°C ± 5°C under an inert atmosphere (argon or nitrogen) for long-term stability. Allow the sealed vial to equilibrate to room temperature in a desiccator before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Retention time corresponds to reference standard
Identification (³¹P NMR)	Spectrum corresponds to reference standard
Purity (HPLC)	≥ 98.0%
Assay (³¹P NMR)	≥ 97.0%
Water Content (KF)	≤ 0.5%
Solubility	Clear, colorless solution in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.