Description

N2-Dmf-5'-o-Dmt-2'-o-Methylguanosine is a key protected nucleoside derivative used as a sophisticated building block in oligonucleotide synthesis. This compound is critical for ensuring high coupling efficiency and sequence fidelity during the automated production of RNA and modified DNA strands. It is primarily required by pharmaceutical R&D teams, biotechnology companies, and CROs engaged in the development of antisense oligonucleotides, siRNA therapeutics, and other advanced nucleic acid-based drugs.

Application

• Solid-Phase Oligonucleotide Synthesis (SPOS): Serves as a phosphoramidite precursor for the controlled incorporation of 2'-O-methylguanosine residues into RNA sequences.
• Therapeutic Oligonucleotide Manufacturing: Essential for the GMP-scale production of siRNA and antisense drug candidates requiring site-specific 2'-O-methyl modifications for enhanced stability and potency.
• Research & Diagnostic Probe Development: Used in synthesizing labeled probes and primers with tailored nuclease resistance and hybridization properties for molecular diagnostics and genomics research.
• Medicinal Chemistry & SAR Studies: A valuable intermediate for exploring structure-activity relationships in novel nucleoside analog drug discovery programs.

Basic Information

Item	Details
Product Name	N2-Dmf-5'-o-Dmt-2'-o-Methylguanosine
CAS No.	128219-76-1
Molecular Formula	C38H43N7O8
Molecular Weight	725.80 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-O-methyl-N2-(dimethylaminomethylene)guanosine; 2'-O-Methyl-N2-(dimethylaminomethylene)-5'-O-(4,4'-dimethoxytrityl)guanosine; N2-DMF-2'-O-methyl-5'-O-DMT-guanosine; DMT-2'-O-Me-G(DMF)-CE Phosphoramidite precursor; Guanosine, N2-[(dimethylamino)methylene]-2'-O-methyl-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-
EINECS	Contact for details

Quality Control

Every batch of N2-Dmf-5'-o-Dmt-2'-o-Methylguanosine is manufactured and tested under strict quality management systems. We provide comprehensive analytical data to ensure the material meets the stringent requirements for oligonucleotide synthesis, including HPLC purity, identity confirmation (NMR, MS), and moisture content analysis. Certificates of Analysis (COA) with lot-specific data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under anhydrous conditions. This material is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. Allow the sealed container to equilibrate to room temperature before opening to minimize moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, DMF, pyridine

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.