Description

5'-o-(4,4'-Dimethoxytrityl)-3'-o-Methyluridine is a key protected nucleoside derivative used as a sophisticated building block in oligonucleotide synthesis. This compound is critical for ensuring high coupling efficiency and purity in the production of modified RNA and DNA sequences. It is primarily required by pharmaceutical R&D teams, biotechnology companies, and CROs engaged in the development of antisense oligonucleotides, siRNA, and other advanced therapeutic nucleic acids.

Application

• Solid-Phase Oligonucleotide Synthesis: Serves as a crucial phosphoramidite precursor for the controlled introduction of 3'-O-methyl modifications into RNA strands.
• Therapeutic Nucleic Acid Development: Used in the research and manufacturing of siRNA, antisense oligonucleotides (ASOs), and aptamers where nuclease resistance and stability are required.
• Probe and Primer Synthesis: Enables the creation of labeled or modified DNA/RNA probes and primers for diagnostic assays and genomic research.
• Chemical Biology Research: A valuable tool for studying RNA structure, function, and protein-RNA interactions through site-specific incorporation.
• Process Development & Scale-Up: Supports process chemistry teams in developing robust and scalable GMP-compliant synthesis routes for nucleic acid therapeutics.

Basic Information

Product Name	5'-o-(4,4'-Dimethoxytrityl)-3'-o-Methyluridine
CAS No.	127212-40-2
Molecular Formula	C32H34N2O8
Molecular Weight	574.62 g/mol
Synonyms	3'-O-Methyl-5'-O-(4,4'-dimethoxytrityl)uridine; 5'-O-DMT-3'-O-methyluridine; 1-[(2R,3R,4R,5R)-5-[[Bis(4-methoxyphenyl)-phenylmethoxy]methyl]-4-methoxy-3-(2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methanol; DMT-3'-O-Methyluridine; N1-((2R,3R,4R,5R)-5-((Bis(4-methoxyphenyl)(phenyl)methoxy)methyl)-4-methoxy-3-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl)methanol
EINECS	Contact for details

Quality Control

Our 5'-o-(4,4'-Dimethoxytrityl)-3'-o-Methyluridine is manufactured under strict quality systems to meet the exacting standards of pharmaceutical and biotechnology applications. Each batch undergoes comprehensive analytical characterization, including HPLC for purity, NMR for structural confirmation, and MS for identity verification. Certificates of Analysis (COA) detailing all specifications are provided and can be tailored to support regulatory filings (e.g., FDA, EMA).

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions or a dry inert atmosphere to maintain stability. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.