Description

3'-Thiothymidine is a modified nucleoside analog where the oxygen atom in the 3'-position of the sugar moiety is replaced by a sulfur atom. This structural modification is critical for its role as a key intermediate in the synthesis of novel oligonucleotides and potential antiviral or anticancer agents. It is primarily utilized by research institutions and pharmaceutical companies engaged in the development of nucleotide-based therapeutics and diagnostic probes. The compound's unique thio-sugar backbone offers enhanced nuclease resistance and altered binding properties, making it valuable for advanced biochemical and medicinal chemistry applications.

Application

• Pharmaceutical Intermediate: A crucial building block for the synthesis of modified antisense oligonucleotides (ASOs) and small interfering RNA (siRNA) therapeutics.
• Antiviral Research: Used in the development of nucleoside analogs targeting viral polymerases for potential treatments against HIV, hepatitis, and other viral infections.
• Cancer Research: Investigated as a precursor for prodrugs and targeted cancer therapies that exploit altered nucleotide metabolism in tumor cells.
• Biochemical Tool: Employed in enzymatic studies to probe the mechanism and specificity of DNA/RNA polymerases and nucleases.
• Diagnostic Probe Development: Incorporated into oligonucleotide probes for enhanced stability in diagnostic assays and hybridization techniques.
• Material Science: Serves as a monomer for creating specialized DNA/RNA analogs with unique physical and chemical properties for nanotechnology applications.

Basic Information

Product Name	3'-Thiothymidine
CAS No.	126862-95-1
Molecular Formula	C10H14N2O4S
Molecular Weight	258.30 g/mol
Synonyms	1-[(2R,4S,5R)-4-Hydroxy-5-(hydroxymethyl)-2-sulfanyltetrahydrofuran-2-yl]-5-methylpyrimidine-2,4-dione; 3'-Thio-2'-deoxythymidine; 3'-S-Thymidine; 2'-Deoxy-3'-thioribothymidine; 3'-Thio-dT; Thymidine, 3'-thio-
EINECS	Contact for details

Quality Control

Our 3'-Thiothymidine is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and tests for residual solvents and moisture content. We provide Certificates of Analysis (COA) with each shipment, detailing all specifications and results. Our quality commitment ensures material suitable for research and development applications in regulated environments.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.