Description

N(4)-(6-Aminohexyl)Cytidine 5'-Triphosphate is a chemically modified nucleotide triphosphate analog featuring a reactive primary amine group attached via a hexyl linker to the cytidine base. This modification is critical for its primary use as a versatile building block in the synthesis of labeled nucleic acids and for covalent attachment to surfaces or other molecules. It is an essential reagent for researchers and developers in the fields of molecular diagnostics, next-generation sequencing (NGS) probe development, and microarray fabrication. The product is supplied as a high-purity, lyophilized solid and is identified by CAS NO 123354-89-2.

Application

• Enzymatic Synthesis of Amino-Modified Nucleic Acids: Used by DNA/RNA polymerases to incorporate amine-functionalized cytidine residues into oligonucleotides for subsequent labeling.
• Probe and Primer Labeling: Serves as the starting point for creating fluorescent, biotinylated, or other chemically tagged probes for hybridization assays (e.g., FISH, microarrays).
• Surface Functionalization: The primary amine group allows for covalent immobilization of oligonucleotides onto activated surfaces (e.g., NHS-ester coated slides, beads) for biosensor and microarray development.
• Cross-linking and Conjugation: Enables the site-specific conjugation of nucleic acids to proteins, peptides, or other biomolecules for therapeutic or diagnostic applications.
• Next-Generation Sequencing (NGS): Utilized in the preparation of custom sequencing libraries and the development of novel sequencing-by-synthesis chemistries.
• Aptamer and SELEX Technology: Incorporated into random nucleic acid libraries to introduce a handle for selection and isolation of target-binding sequences.

Basic Information

Product Name	N(4)-(6-Aminohexyl)Cytidine 5'-Triphosphate
CAS No.	123354-89-2
Molecular Formula	C16H28N6O13P3
Molecular Weight	638.36 g/mol
Synonyms	6-(N-Cytidin-4-yl)aminohexyl triphosphate; Aminohexyl-dCTP; AH-dCTP; N4-(6-Aminohexyl)-2'-deoxycytidine 5'-triphosphate; 5'-ATP, N-[6-[(4-amino-2-oxo-1(2H)-pyrimidinyl)methyl]amino]hexyl]-; dCTP-6-NH2; 2'-Deoxy-N4-(6-aminohexyl)cytidine-5'-triphosphate
EINECS	Contact for details

Quality Control

Our N(4)-(6-Aminohexyl)Cytidine 5'-Triphosphate is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity, suitable for sensitive biochemical applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We ensure compliance with relevant industry standards for molecular biology-grade reagents.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This compound is hygroscopic (moisture-sensitive). For long-term storage, keep under an inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white lyophilized solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Biological Activity	Suitable for enzymatic incorporation

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.