Description

2'-O-TBDMS-5'-O-DMT-N4-Ac Cytidin is a key protected nucleoside phosphoramidite building block, essential for the automated solid-phase synthesis of oligonucleotides. Its primary value lies in enabling the precise and efficient incorporation of cytidine into DNA and RNA sequences, which is critical for research and therapeutic development. This high-purity intermediate is indispensable for biotechnology and pharmaceutical companies engaged in antisense therapy, siRNA development, PCR primers, and diagnostic probe manufacturing.

Application

• Oligonucleotide Synthesis: As a critical phosphoramidite monomer for the automated synthesis of DNA and RNA strands.
• Antisense Oligonucleotide (ASO) Production: For the development of therapeutic agents that target specific RNA sequences.
• siRNA and miRNA Synthesis: Enabling the manufacture of RNA interference (RNAi) tools for gene silencing research and therapeutics.
• PCR Primer and Probe Manufacture: Used in creating high-fidelity primers and labeled probes for molecular diagnostics and genetic testing.
• Research & Development: Serving as a fundamental reagent in academic and industrial labs for nucleic acid chemistry and biochemistry studies.
• Synthesis of Modified Oligonucleotides: For creating oligonucleotides with tailored properties for enhanced stability, binding affinity, or delivery.

Basic Information

Product Name	2'-O-TBDMS-5'-O-DMT-N4-Ac Cytidin
CAS No.	121058-85-3
Molecular Formula	C41H53N3O8Si
Molecular Weight	743.97 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-O-(tert-butyldimethylsilyl)-N4-acetylcytidine; N4-Acetyl-5'-O-(4,4'-dimethoxytrityl)-2'-O-[(1,1-dimethylethyl)dimethylsilyl]cytidine; 2'-O-TBDMS-5'-O-DMT-N4-Acetylcytidine; DMT-rC(Ac)-TBDMS Amidite Precursor; Protected Cytidine Phosphoramidite Synthon; N4-Ac-5'-DMT-2'-TBDMS-Cytidine
EINECS	Contact for details

Quality Control

Our 2'-O-TBDMS-5'-O-DMT-N4-Ac Cytidin is manufactured under strict quality systems to ensure the high purity and consistency required for sensitive oligonucleotide synthesis. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and critical impurity profiles. We adhere to industry-best practices, and our quality commitment supports applications in GMP-grade therapeutic oligonucleotide development.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This compound is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions or a dry inert atmosphere to maintain stability and purity. For long-term storage, consider storing desiccated at -20°C.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.