Description

2'-Tbdms-Ac-Rc CAS NO 119794-51-3 is a high-purity, protected nucleoside phosphoramidite derivative, a critical building block in the automated solid-phase synthesis of oligonucleotides. Its primary value lies in enabling the precise and efficient incorporation of modified ribonucleotides into RNA sequences, which is essential for advanced research and therapeutic development. This compound is indispensable for pharmaceutical R&D teams, diagnostic developers, and academic researchers working on siRNA, antisense oligonucleotides, mRNA therapeutics, and molecular probes.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite monomer for the solid-phase synthesis of RNA and modified RNA strands.
• Therapeutic RNA Development: Critical raw material in the production of siRNA (small interfering RNA), antisense oligonucleotides (ASOs), and mRNA for therapeutic applications.
• Diagnostic Probe Manufacture: Used to synthesize labeled probes for diagnostic assays, including qPCR and FISH (Fluorescence In Situ Hybridization).
• Research & Development: Enables the study of RNA structure, function, and interactions by allowing site-specific modifications.
• Apitamer Production: Serves as a building block in the synthesis of modified aptamers for targeted drug delivery and sensing.
• CRISPR Guide RNA Synthesis: Used in the preparation of chemically modified guide RNAs (gRNAs) to enhance stability and efficacy in gene-editing applications.

Basic Information

Product Name	2'-Tbdms-Ac-Rc
CAS No.	119794-51-3
Molecular Formula	C₄₀H₆₇N₄O₈PSi
Molecular Weight	791.04 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-O-[(tert-butyldimethylsilyl)]-N4-acetylcytidine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 2'-O-TBDMS-N4-Acetylcytidine Phosphoramidite; N4-Ac-2'-O-TBDMS-C Phosphoramidite; 2'-O-TBDMS-N4-Ac-C Phosphoramidite; 2'-TBDMS-Ac-C Phosphoramidite; 2'-O-(tert-Butyldimethylsilyl)-N4-acetylcytidine 3'-(2-cyanoethyl diisopropylphosphoramidite); Ac-rC(TBDMS) Phosphoramidite
EINECS	Contact for details

Quality Control

Every batch of 2'-Tbdms-Ac-Rc is manufactured under strict quality systems and undergoes comprehensive analytical testing to ensure it meets the exacting standards required for oligonucleotide synthesis. We provide a detailed Certificate of Analysis (COA) with each shipment, which includes data for purity (HPLC), identity (NMR, MS), moisture content (KF), and phosphoramidite assay. Our quality commitment aligns with cGMP principles for pharmaceutical starting materials, ensuring reliable performance and batch-to-batch consistency for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, freezer. This compound is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Moisture (KF)	≤ 1.0%
Assay (³¹P NMR)	≥ 95.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.