Description

3'-Azido-3'-Deoxythymidine-Methyl-3H CAS NO 118897-00-0 is a tritium-labeled derivative of the nucleoside analog Azidothymidine (AZT). This compound is a critical tool in pharmaceutical research and development, particularly for studying the metabolism, distribution, and pharmacokinetics of AZT. It is primarily utilized by research scientists and analytical laboratories in the pharmaceutical, biotechnology, and academic sectors for advanced tracer studies and drug discovery programs.

Application

• Metabolic Pathway Tracing: Used as a radiolabeled tracer to study the in vitro and in vivo metabolic fate of Azidothymidine (AZT).
• Pharmacokinetic (PK) Studies: Essential for quantitative analysis of drug absorption, distribution, metabolism, and excretion (ADME) in preclinical research.
• Drug Discovery & Development: Supports mechanism-of-action studies and the development of new antiviral and antiretroviral agents.
• Biochemical Assays: Employed in receptor binding studies, enzyme inhibition assays, and other investigative biochemical techniques.
• Analytical Reference Standard: Serves as a high-purity internal standard for Liquid Chromatography-Mass Spectrometry (LC-MS) and other sensitive analytical methods.
• Academic Research: Used in university and institutional labs for fundamental virology and nucleoside chemistry research.

Basic Information

Product Name	3'-Azido-3'-Deoxythymidine-Methyl-3H
CAS No.	118897-00-0
Molecular Formula	C11H14N5O5 (3H)
Molecular Weight	~286.26 g/mol (unlabeled)
Synonyms	AZT-Methyl-3H; [Methyl-3H]AZT; 3'-Azido-3'-deoxythymidine (methyl-3H); Tritiated AZT; Zidovudine-methyl-tritium; Azidothymidine-methyl-3H; BW A509U (tritiated); 3'-Azido-2',3'-dideoxythymidine-methyl-3H
EINECS	Contact for details

Quality Control

Every batch of 3'-Azido-3'-Deoxythymidine-Methyl-3H is manufactured and handled under strict quality protocols to ensure the highest standards of radiochemical purity and specific activity. Our products undergo rigorous quality testing, including analytical HPLC and radiometric detection, to ensure compliance with research-grade standards. A comprehensive Certificate of Analysis (COA) detailing specific activity, radiochemical purity, chemical purity, and solvent composition is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at or below -20°C in the original solvent as specified on the label and COA. This product is hygroscopic (moisture-sensitive); ensure containers are sealed under inert atmosphere after each use to maintain stability and prevent decomposition. For long-term storage, keep in a dedicated, well-ventilated freezer designated for radioactive materials.

Specification

Item	Specification
Appearance	Clear solution (color may vary)
Chemical Purity (HPLC)	≥ 95%
Radiochemical Purity	≥ 97%
Specific Activity	10-80 Ci/mmol (Contact for exact value)
Identification (HPLC co-elution)	Conforms to reference standard
Solvent	Ethanol, Toluene, or custom (as specified)
Concentration	1.0 mCi/mL (Nominal)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.