Description

5'-o-(4,4'-Dimethoxytriphenylmethyl)Dithymidyl (3',5')-4,4'-Dimethoxytriphenylmethanephosphonate is a specialized phosphoramidite monomer, a critical building block in the automated solid-phase synthesis of oligonucleotides. Its primary value lies in enabling the precise and efficient incorporation of thymidine nucleotides, ensuring high coupling yields and sequence fidelity. This compound is essential for research and production in pharmaceutical development, molecular diagnostics, and synthetic biology, where reliable, high-purity reagents are non-negotiable.

Application

• Oligonucleotide Synthesis: As a key thymidine phosphoramidite monomer in automated DNA/RNA synthesizers for research-scale and GMP production.
• Therapeutic Oligonucleotide Manufacturing: Production of antisense oligonucleotides, siRNA, and aptamers for drug discovery and development.
• Diagnostic Probe Synthesis: Fabrication of labeled and unlabeled DNA probes for PCR, qPCR, FISH, and microarray applications.
• Primer and Gene Fragment Construction: Synthesis of primers for sequencing, cloning, and gene assembly techniques.
• Synthetic Biology: Building custom genetic circuits and synthetic genes for metabolic engineering and basic research.
• Bioconjugation and Labeling: Serving as a starting material for the synthesis of modified nucleotides with functional tags (e.g., fluorophores, biotin).

Basic Information

Product Name	5'-o-(4,4'-Dimethoxytriphenylmethyl)Dithymidyl (3',5')-4,4'-Dimethoxytriphenylmethanephosphonate
CAS No.	118867-43-9
Molecular Formula	C58H59N4O13P
Molecular Weight	1050.07 g/mol
Synonyms	DMT-dT Phosphoramidite; Thymidine 3'-Phosphoramidite, DMT-protected; 5'-O-(4,4'-Dimethoxytrityl)thymidine 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; dT Phosphoramidite; DMT-Thymidine Phosphoramidite; NCI-Protected Thymidine Phosphoramidite; Amidite dT; CAS 118867-43-9
EINECS	Contact for details

Quality Control

Every batch of our phosphoramidite monomers is produced and tested under strict quality management systems to ensure consistency and reliability for sensitive oligonucleotide synthesis. We provide comprehensive Certificates of Analysis (COA) detailing purity (HPLC), identity (NMR, IR), and moisture content (Karl Fischer). Our quality protocols are designed to meet the exacting standards required for research and cGMP-grade manufacturing.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at -20°C under an inert atmosphere (argon or nitrogen) due to its hygroscopic and moisture-sensitive nature. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Moisture Content (KF)	≤ 0.5%
Solubility	Clear, colorless solution in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.