Description

Cytidine-5'-o-(1-Thiotriphosphate), Rp-Isomer Sodium Salt is a chemically modified nucleotide analog where a non-bridging oxygen atom in the α-phosphate group is replaced by sulfur, specifically in the Rp stereochemical configuration. This precise modification makes it a critical tool for studying enzyme mechanisms, particularly as a substrate or inhibitor for ATP-dependent enzymes like kinases and polymerases, where it resists hydrolysis. It is essential for researchers and manufacturers in biochemical research, pharmaceutical development, and molecular diagnostics who require high-purity, stereochemically defined compounds for mechanistic studies and assay development.

Application

• Enzyme Kinetics and Mechanistic Studies: Used as a hydrolysis-resistant ATP analog to probe the catalytic mechanisms of kinases, ATPases, and other phosphotransferase enzymes.
• Nucleic Acid Research: Employed in studies of RNA polymerase and reverse transcriptase activity, helping to understand transcription and replication fidelity.
• Pharmaceutical Screening: Serves as a key component in high-throughput screening assays to identify potential inhibitors of ATP-binding proteins involved in disease pathways.
• Structural Biology: Utilized in X-ray crystallography and NMR studies to capture snapshots of enzyme-substrate complexes, aiding in rational drug design.
• Diagnostic Assay Development: Incorporated into specialized diagnostic kits that rely on nucleotide-dependent enzymatic reactions for signal amplification.
• Biochemical Tool Manufacturing: Supplied as a raw material for companies producing specialized reagent kits for life science research.

Basic Information

Product Name	Cytidine-5'-o-(1-Thiotriphosphate), Rp-Isomer Sodium Salt
CAS No.	118353-34-7
Molecular Formula	C9H14N3O13P3S • xNa+
Molecular Weight	Approx. 527.1 (Free acid) - Contact for exact sodium salt form weight.
Synonyms	CTPαS, Rp isomer; Rp-CTP-α-S; Cytidine-5'-triphosphate-α-thiol, Rp isomer sodium salt; Adenosine 5'-(γ-thiotriphosphate) Rp-isomer (related analog); Sp-1-Thio-CTP (diastereomer); 5'-CTP-α-S, Rp; Nucleotide thiotriphosphate analog; Rp-Cytidine-5'-O-(1-thiotriphosphate)
EINECS	Contact for details.

Quality Control

Our Cytidine-5'-o-(1-Thiotriphosphate), Rp-Isomer Sodium Salt is produced and controlled under a strict quality management system. Each batch undergoes rigorous analytical testing including HPLC for purity and diastereomeric excess, NMR for structural confirmation, and mass spectrometry. We provide comprehensive Certificates of Analysis (COA) with detailed chromatographic data, ensuring traceability and compliance with research-grade specifications. Our quality commitment supports applications requiring the highest levels of stereochemical purity and batch-to-batch consistency.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive). For long-term stability, keep the container sealed under an inert atmosphere and avoid repeated freeze-thaw cycles. Handle with appropriate personal protective equipment in a well-ventilated area.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Diastereomeric Purity (Rp-Isomer)	≥ 98%
Water Content (KF)	≤ 3.0%
Solubility	Soluble in water, buffer solutions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.