Description

Adenosine 5'-Triphosphate P3-[1-(2-Nitrophenyl)Ethyl Ester] Trisodium Salt is a chemically caged derivative of ATP, a fundamental molecule for energy transfer in biological systems. This compound is specifically engineered to release biologically active ATP upon photolysis, providing researchers with precise temporal and spatial control over ATP concentration in complex biochemical and cellular systems. It is an essential tool for scientists in neurobiology, muscle physiology, and cell signaling research, enabling the study of ATP-dependent processes with high precision.

Application

• Photolytic Release Studies: Used as a "caged" ATP precursor for rapid, light-triggered release of ATP in kinetic experiments.
• Cellular Physiology Research: Investigating ATP-gated ion channels (P2X receptors) and purinergic signaling pathways in real-time.
• Muscle Biophysics: Studying the kinetics of actomyosin cross-bridge cycling and muscle contraction mechanisms.
• Neuroscience: Probing the role of ATP as a co-transmitter or modulator in synaptic transmission.
• Enzyme Kinetics: A substrate for time-resolved studies of ATP-dependent enzymes like kinases, ATPases, and synthases.
• Intracellular Signaling: Mapping localized energy demands and signaling events within living cells.
• Drug Discovery Screening: A tool for developing and testing compounds that target ATP-binding sites or ATP-utilizing processes.

Basic Information

Item	Detail
Product Name	Adenosine 5'-Triphosphate P3-[1-(2-Nitrophenyl)Ethyl Ester] Trisodium Salt
CAS No.	117961-29-2
Molecular Formula	C20H23N6O16P3Na3
Molecular Weight	789.30 g/mol
Synonyms	P3-[1-(2-Nitrophenyl)ethyl] ATP Trisodium Salt; Caged ATP (2-Nitrophenylethyl ester); NPE-caged ATP; ATP, P3-[1-(2-nitrophenyl)ethyl] ester, trisodium salt; Adenosine-5'-triphosphate P3-(1-(2-nitrophenyl)ethyl) ester trisodium salt; "Caged" Adenosine Triphosphate; Photolabile ATP analog; 1-(2-Nitrophenyl)ethyl-caged ATP
EINECS	Contact for details

Quality Control

Our caged ATP is produced under stringent conditions to ensure high chemical purity and reliable photolytic performance. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard photolysis assays. We adhere to rigorous quality testing protocols suitable for research and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term stability, store desiccated. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Extinction Coefficient (ε)	~5,200 M⁻¹cm⁻¹ at 260 nm (for ATP moiety)
Photolytic Efficiency	Contact for details
Water Content (KF)	≤ 5.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.